Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

Arnaud Javelle, Blanca-Rosa Rodríguez-Pastrana, Christophe Jacob, Bernard Botton, Annick Brun, Bruno André, Anne-Marie Marini, Michel Chalot

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.

Original languageEnglish
Pages (from-to)393-8
Number of pages6
JournalFEBS Letters
Volume505
Issue number3
DOIs
Publication statusPublished - 21 Sep 2001

Keywords

  • ammonium transport
  • base sequence
  • basidiomycota
  • carrier proteins
  • cloning molecular
  • DNA primers
  • gene expression regulation fungal
  • kinetics
  • protein isoforms
  • saccharomyces cerevisiae
  • methylamine
  • nitrogen regulation
  • hebeloma cylindrosporum

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