Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance

Miriam Strosova, Jana Karlovska, C.M. Spickett, Zuzana Orszagova, Silvester Ponist, Katarina Bauerova, Danica Mihalova, Lubica Horakova

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adjuvant arthritis (AA) is a condition that involves systemic oxidative stress. Unexpectedly, it was found that sarcoplasmic reticulum Ca2 +-ATPase (SERCA) activity was elevated in muscles of rats with AA compared to controls, suggesting possible conformational changes in the enzyme. There was no alteration in the nucleotide binding site but rather in the transmembrane domain according to the tryptophan polar/non-polar fluorescence ratio. Higher relative expression of SERCA, higher content of nitrotyrosine but no increase in phospholipid oxidation in AA SR was found. In vitro treatments of SR with HOCl showed that in AA animals SERCA activity was more susceptible to oxidative stress, but SR phospholipids were more resistant and SERCA could also be activated by phosphatidic acid. It was concluded that increased SERCA activity in AA was due to increased levels of SERCA protein and structural changes to the protein, probably induced by direct and specific oxidation involving reactive nitrogen species.
LanguageEnglish
Pages852-864
Number of pages12
JournalFree Radical Research
Volume43
Issue number9
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Experimental Arthritis
Oxidative stress
Oxidation-Reduction
Phospholipids
Modulation
Reactive Nitrogen Species
Oxidation
Phosphatidic Acids
Calcium-Transporting ATPases
Tryptophan
Muscle
Rats
Animals
Proteins
Nucleotides
Fluorescence
Binding Sites
Oxidative Stress
Enzymes
Sarcoplasmic Reticulum

Keywords

  • adjuvant arthritis
  • oxidative stress
  • Ca2 +-ATPase
  • sarcoplasmic reticulum
  • nitrotyrosine

Cite this

Strosova, M., Karlovska, J., Spickett, C. M., Orszagova, Z., Ponist, S., Bauerova, K., ... Horakova, L. (2009). Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance. Free Radical Research, 43(9), 852-864. https://doi.org/10.1080/10715760903089708
Strosova, Miriam ; Karlovska, Jana ; Spickett, C.M. ; Orszagova, Zuzana ; Ponist, Silvester ; Bauerova, Katarina ; Mihalova, Danica ; Horakova, Lubica. / Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance. In: Free Radical Research. 2009 ; Vol. 43, No. 9. pp. 852-864.
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Strosova, M, Karlovska, J, Spickett, CM, Orszagova, Z, Ponist, S, Bauerova, K, Mihalova, D & Horakova, L 2009, 'Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance' Free Radical Research, vol. 43, no. 9, pp. 852-864. https://doi.org/10.1080/10715760903089708

Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance. / Strosova, Miriam; Karlovska, Jana; Spickett, C.M.; Orszagova, Zuzana; Ponist, Silvester; Bauerova, Katarina; Mihalova, Danica; Horakova, Lubica.

In: Free Radical Research, Vol. 43, No. 9, 09.2009, p. 852-864.

Research output: Contribution to journalArticle

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