Modulation of protein-surface interactions on nanopatterned polymer films

K. H. A. Lau, J. Bang, C. J. Hawker, D. H. Kim, W. Knoll

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The introduction of nanoscale features brings with it a high density of surface interface boundaries and effectively introduces an additional boundary material that exhibits properties different from the surrounding surfaces. We systematically varied the feature size of self-assembled polystyrene-block-poly(methyl methacrylate) copolymer nanopatterns from 13 to 200 am and demonstrated that the basic property of protein adsorption on a nanopatterned surface can be modulated by the length density of surface interfaces present. Protein adsorption on the nanopatterns could be described by a modified adsorption affinity along the surface interface with an effective width on the length-scale of individual proteins. Due to the intrinsic high density of surface interfaces in many polymeric thin film nanopatterns and structures, the interaction of proteins with such interfaces may be of particular relevance to cell-surface studies and to biomaterial and biosensor applications involving nanoscale features.
LanguageEnglish
Pages1061-1066
Number of pages6
JournalBiomacromolecules
Volume10
Issue number5
DOIs
Publication statusPublished - 20 Mar 2009

Fingerprint

Polymer films
Membrane Proteins
Modulation
Proteins
Adsorption
Biocompatible Materials
Polymethyl methacrylates
Biosensors
Biomaterials
Polystyrenes
Copolymers
Thin films

Keywords

  • orientation activation
  • atomic-force microscopy
  • thin-films
  • monoclonal-antibody
  • copolymer templates
  • diblock copolymers
  • block-copolymers
  • adsorption nanoarrays

Cite this

Lau, K. H. A. ; Bang, J. ; Hawker, C. J. ; Kim, D. H. ; Knoll, W. / Modulation of protein-surface interactions on nanopatterned polymer films. In: Biomacromolecules. 2009 ; Vol. 10, No. 5. pp. 1061-1066.
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Modulation of protein-surface interactions on nanopatterned polymer films. / Lau, K. H. A.; Bang, J.; Hawker, C. J.; Kim, D. H.; Knoll, W.

In: Biomacromolecules, Vol. 10, No. 5, 20.03.2009, p. 1061-1066.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modulation of protein-surface interactions on nanopatterned polymer films

AU - Lau, K. H. A.

AU - Bang, J.

AU - Hawker, C. J.

AU - Kim, D. H.

AU - Knoll, W.

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AB - The introduction of nanoscale features brings with it a high density of surface interface boundaries and effectively introduces an additional boundary material that exhibits properties different from the surrounding surfaces. We systematically varied the feature size of self-assembled polystyrene-block-poly(methyl methacrylate) copolymer nanopatterns from 13 to 200 am and demonstrated that the basic property of protein adsorption on a nanopatterned surface can be modulated by the length density of surface interfaces present. Protein adsorption on the nanopatterns could be described by a modified adsorption affinity along the surface interface with an effective width on the length-scale of individual proteins. Due to the intrinsic high density of surface interfaces in many polymeric thin film nanopatterns and structures, the interaction of proteins with such interfaces may be of particular relevance to cell-surface studies and to biomaterial and biosensor applications involving nanoscale features.

KW - orientation activation

KW - atomic-force microscopy

KW - thin-films

KW - monoclonal-antibody

KW - copolymer templates

KW - diblock copolymers

KW - block-copolymers

KW - adsorption nanoarrays

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