Modulation of dopamine efflux in the nucleus accumbens after cholinergic stimulation of the ventral tegmental area in intact, pedunculopontine tegmental nucleus-lesioned, and laterodorsal tegmental nucleus-lesioned rats

Charles D. Blaha, Laura F. Allen, Sheela Das, Wendy L. Inglis, Mary P. Latimer, Steven R. Vincent, Philip Winn

Research output: Contribution to journalArticle

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Abstract

Microinjections of the cholinergic receptor agonist nicotine and the cholinesterase inhibitor neostigmine were made into the ventral tegmental area (VTA) of urethane-anesthetized rats, and dopamine (DA) efflux in the nucleus accumbens was measured using in vivo chronoamperometry. Dose- dependent increases in the chronoamperometric signals corresponding to increased DA efflux were observed in the nucleus accumbens of normal intact rats after cholinergic stimulation of the VTA. The source of the cholinergic input to the VTA was investigated by making excitotoxic lesions in either the laterodorsal tegmental nucleus (LDTg) or the pedunculopontine tegmental nucleus (PPTg). Compared with sham operated control animals, which showed the same response as intact, nonlesioned rats, ibotenate lesions of the LDTg attenuated the stimulatory effects of intra-VTA neostigmine on DA efflux in the nucleus accumbens. In contrast, rats with ibotenate lesions of the PPTg showed normal nucleus accumbens DA efflux after intra-VTA injections of neostigmine. Such lesions in the PPTg attenuate DA efflux in the caudate- putamen stimulated by injections of neostigmine into the substantia nigra pars compacta (SNc). The present data show that cholinergic neurons in the LDTg, but not the PPTg, regulate the activity of DA-containing neurons in the VTA, which complements previous data showing that cholinergic neurons in the PPTg regulate DA-containing neurons in the SNc.

LanguageEnglish
Pages714-722
Number of pages9
JournalJournal of Neuroscience
Volume16
Issue number2
DOIs
Publication statusPublished - 15 Jan 1996

Fingerprint

Pedunculopontine Tegmental Nucleus
Ventral Tegmental Area
Nucleus Accumbens
Cholinergic Agents
Neostigmine
Dopamine
Cholinergic Neurons
Dopaminergic Neurons
Cholinergic Agonists
Injections
Putamen
Urethane
Cholinesterase Inhibitors
Microinjections
Cholinergic Receptors
Nicotine

Keywords

  • acetylcholine
  • chronoamperometry
  • dopamine
  • ibotenate
  • laterodorsal tegmental nucleus
  • nucleus accumbens
  • pedunculopontine tegmental nucleus
  • rat
  • ventral tegmental area

Cite this

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title = "Modulation of dopamine efflux in the nucleus accumbens after cholinergic stimulation of the ventral tegmental area in intact, pedunculopontine tegmental nucleus-lesioned, and laterodorsal tegmental nucleus-lesioned rats",
abstract = "Microinjections of the cholinergic receptor agonist nicotine and the cholinesterase inhibitor neostigmine were made into the ventral tegmental area (VTA) of urethane-anesthetized rats, and dopamine (DA) efflux in the nucleus accumbens was measured using in vivo chronoamperometry. Dose- dependent increases in the chronoamperometric signals corresponding to increased DA efflux were observed in the nucleus accumbens of normal intact rats after cholinergic stimulation of the VTA. The source of the cholinergic input to the VTA was investigated by making excitotoxic lesions in either the laterodorsal tegmental nucleus (LDTg) or the pedunculopontine tegmental nucleus (PPTg). Compared with sham operated control animals, which showed the same response as intact, nonlesioned rats, ibotenate lesions of the LDTg attenuated the stimulatory effects of intra-VTA neostigmine on DA efflux in the nucleus accumbens. In contrast, rats with ibotenate lesions of the PPTg showed normal nucleus accumbens DA efflux after intra-VTA injections of neostigmine. Such lesions in the PPTg attenuate DA efflux in the caudate- putamen stimulated by injections of neostigmine into the substantia nigra pars compacta (SNc). The present data show that cholinergic neurons in the LDTg, but not the PPTg, regulate the activity of DA-containing neurons in the VTA, which complements previous data showing that cholinergic neurons in the PPTg regulate DA-containing neurons in the SNc.",
keywords = "acetylcholine, chronoamperometry, dopamine, ibotenate, laterodorsal tegmental nucleus, nucleus accumbens, pedunculopontine tegmental nucleus, rat, ventral tegmental area",
author = "Blaha, {Charles D.} and Allen, {Laura F.} and Sheela Das and Inglis, {Wendy L.} and Latimer, {Mary P.} and Vincent, {Steven R.} and Philip Winn",
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Modulation of dopamine efflux in the nucleus accumbens after cholinergic stimulation of the ventral tegmental area in intact, pedunculopontine tegmental nucleus-lesioned, and laterodorsal tegmental nucleus-lesioned rats. / Blaha, Charles D.; Allen, Laura F.; Das, Sheela; Inglis, Wendy L.; Latimer, Mary P.; Vincent, Steven R.; Winn, Philip.

In: Journal of Neuroscience, Vol. 16, No. 2, 15.01.1996, p. 714-722.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Modulation of dopamine efflux in the nucleus accumbens after cholinergic stimulation of the ventral tegmental area in intact, pedunculopontine tegmental nucleus-lesioned, and laterodorsal tegmental nucleus-lesioned rats

AU - Blaha, Charles D.

AU - Allen, Laura F.

AU - Das, Sheela

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AU - Latimer, Mary P.

AU - Vincent, Steven R.

AU - Winn, Philip

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AB - Microinjections of the cholinergic receptor agonist nicotine and the cholinesterase inhibitor neostigmine were made into the ventral tegmental area (VTA) of urethane-anesthetized rats, and dopamine (DA) efflux in the nucleus accumbens was measured using in vivo chronoamperometry. Dose- dependent increases in the chronoamperometric signals corresponding to increased DA efflux were observed in the nucleus accumbens of normal intact rats after cholinergic stimulation of the VTA. The source of the cholinergic input to the VTA was investigated by making excitotoxic lesions in either the laterodorsal tegmental nucleus (LDTg) or the pedunculopontine tegmental nucleus (PPTg). Compared with sham operated control animals, which showed the same response as intact, nonlesioned rats, ibotenate lesions of the LDTg attenuated the stimulatory effects of intra-VTA neostigmine on DA efflux in the nucleus accumbens. In contrast, rats with ibotenate lesions of the PPTg showed normal nucleus accumbens DA efflux after intra-VTA injections of neostigmine. Such lesions in the PPTg attenuate DA efflux in the caudate- putamen stimulated by injections of neostigmine into the substantia nigra pars compacta (SNc). The present data show that cholinergic neurons in the LDTg, but not the PPTg, regulate the activity of DA-containing neurons in the VTA, which complements previous data showing that cholinergic neurons in the PPTg regulate DA-containing neurons in the SNc.

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KW - nucleus accumbens

KW - pedunculopontine tegmental nucleus

KW - rat

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