Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes

Sami Nummelin, Markus Selin, Sacha Legrand, Jarmo Ropponen, Jani Seitsonen, Antti Nykänen, Jari Koivisto, Jouni Hirvonen, Mauri A Kostiainen, Luis M. Bimbo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Materials and methods aimed at the next generation of nanoscale carriers for drugs and other therapeutics are currently in great demand. Yet, creating these precise molecular arrangements in a feasible and straightforward manner represents a remarkable challenge. Herein we report a modular synthetic route for amphiphilic Janus-dendrimers via a copper-catalyzed click reaction (CuAAC) and a facile procedure, using simple injection, to obtain highly uniform dendrimersomes with efficient loading of the model drug compound propranolol. The resulting assemblies were analyzed by dynamic light scattering and cryogenic transmission electronic microscopy revealing the formation of unilamellar and multilamellar dendrimersomes. The formation of a bilayer structure was confirmed using cryo-TEM and confocal microscopy visualization of an encapsulated solvatochromic dye (Nile red). The dendrimersomes reported here are tunable in size, stable over time and display robust thermal stability in aqueous media. Our results expand the scope of dendrimer-based supramolecular colloidal systems and offer the means for one-step fabrication of drug-loaded dendrimersomes in the size range of 90–200 nm, ideal for biomedical applications.
LanguageEnglish
Pages7189-7198
Number of pages9
JournalNanoscale
Volume9
Issue number21
Early online date8 May 2017
DOIs
Publication statusPublished - 1 Jun 2017

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Dendrimers
Drug Compounding
Drug Carriers
Confocal microscopy
Dynamic light scattering
Confocal Microscopy
Propranolol
Pharmaceutical Preparations
Cryogenics
Copper
Microscopy
Microscopic examination
Thermodynamic stability
Coloring Agents
Visualization
Dyes
Hot Temperature
Display devices
Transmission electron microscopy
Fabrication

Keywords

  • drug loaded dendrimersomes
  • nanoscale carriers
  • dendrimersomes

Cite this

Nummelin, Sami ; Selin, Markus ; Legrand, Sacha ; Ropponen, Jarmo ; Seitsonen, Jani ; Nykänen, Antti ; Koivisto, Jari ; Hirvonen, Jouni ; Kostiainen, Mauri A ; Bimbo, Luis M. / Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. In: Nanoscale. 2017 ; Vol. 9, No. 21. pp. 7189-7198.
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Nummelin, S, Selin, M, Legrand, S, Ropponen, J, Seitsonen, J, Nykänen, A, Koivisto, J, Hirvonen, J, Kostiainen, MA & Bimbo, LM 2017, 'Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes' Nanoscale, vol. 9, no. 21, pp. 7189-7198. https://doi.org/10.1039/C6NR08102A

Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. / Nummelin, Sami; Selin, Markus; Legrand, Sacha; Ropponen, Jarmo; Seitsonen, Jani; Nykänen, Antti; Koivisto, Jari; Hirvonen, Jouni; Kostiainen, Mauri A; Bimbo, Luis M.

In: Nanoscale, Vol. 9, No. 21, 01.06.2017, p. 7189-7198.

Research output: Contribution to journalArticle

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T1 - Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes

AU - Nummelin, Sami

AU - Selin, Markus

AU - Legrand, Sacha

AU - Ropponen, Jarmo

AU - Seitsonen, Jani

AU - Nykänen, Antti

AU - Koivisto, Jari

AU - Hirvonen, Jouni

AU - Kostiainen, Mauri A

AU - Bimbo, Luis M.

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N2 - Materials and methods aimed at the next generation of nanoscale carriers for drugs and other therapeutics are currently in great demand. Yet, creating these precise molecular arrangements in a feasible and straightforward manner represents a remarkable challenge. Herein we report a modular synthetic route for amphiphilic Janus-dendrimers via a copper-catalyzed click reaction (CuAAC) and a facile procedure, using simple injection, to obtain highly uniform dendrimersomes with efficient loading of the model drug compound propranolol. The resulting assemblies were analyzed by dynamic light scattering and cryogenic transmission electronic microscopy revealing the formation of unilamellar and multilamellar dendrimersomes. The formation of a bilayer structure was confirmed using cryo-TEM and confocal microscopy visualization of an encapsulated solvatochromic dye (Nile red). The dendrimersomes reported here are tunable in size, stable over time and display robust thermal stability in aqueous media. Our results expand the scope of dendrimer-based supramolecular colloidal systems and offer the means for one-step fabrication of drug-loaded dendrimersomes in the size range of 90–200 nm, ideal for biomedical applications.

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Nummelin S, Selin M, Legrand S, Ropponen J, Seitsonen J, Nykänen A et al. Modular synthesis of self-assembling Janus-dendrimers and facile preparation of drug-loaded dendrimersomes. Nanoscale. 2017 Jun 1;9(21):7189-7198. https://doi.org/10.1039/C6NR08102A