Modular microscopy (ModMicro) solution for volumetric epifluorescence microscopic imaging

Robert Archibald; Harriet  Bishop; Graham M. Gibson; Akhil Kallepalli

doi:10.21203/rs.3.rs-3293259/v1

Modular microscopy (ModMicro) solution for volumetric epifluorescence microscopic imaging

Robert Archibald, Harriet Bishop, Graham M. Gibson, Akhil Kallepalli

Biomedical Engineering

Research output: Working paper › Working Paper/Preprint

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Abstract

Fluorescence microscopy is a well-known technique for studying biological samples. However, on a global scale, microscopy techniques (beyond brightfield imaging) are inaccessible in some countries. Additionally, proprietary microscopy hardware is not modular and cannot be adapted for different architectures. In this research, we demonstrate a modular epifluorescence microscopy system and image analysis technique developed with the motivation of achieving high-resolution imaging using low-cost modular hardware. We compare the performance of the (1000 GBP) OpenFlexure-inspired epifluorescence ModMicro system to a proprietary epifluorescence microscope. With the epifluorescence ModMicro system, we imaged 'datacubes' of 0.54 x 0.40 x 0.005 mm (in 100 steps of 50 nm each) size. The image data is enhanced with a novel sharpening algorithm. Combined (light sources, microscope hardware and image sharpness enhancement algorithm), a complete system for epifluorescence microscopy that is globally accessible and cost-effective is presented.

Original language	English
Number of pages	11
DOIs	https://doi.org/10.21203/rs.3.rs-3293259/v1
Publication status	Published - 30 Aug 2023

Funding

We wish to acknowledge the support of the Leverhulme Trust Early Career Fellowship, EPSRC Impact Acceleration Account(IAA) awards [EP/X5257161/1, EP/R511705/1], EPSRC funding to QuantIC [EP/M01326X/1], University of GlasgowKnowledge Exchange Fund (GKEF), Institute of Physics (IOP), IEEE Photonics Society Seed Grant and the Royal Society. We would like to also thank the incredible assistance and support from Dr Jonathan Taylor, Daniel Olesker and Conall Thompson(Imaging Concepts Group, School of Physics and Astronomy, University of Glasgow) with sample preparation and access tothe Nikon Ti-Eclipse microscope.

Keywords

Fluorescence imaging
microscopy
imaging techniques
applied optics
biophotonics
imaging and sensing

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10.21203/rs.3.rs-3293259/v1Licence: CC BY 4.0

Modular_microscopy_(ModMicro)_solution_for_volumetric_epifluorescence-microscopic_imagingFinal published version, 5.41 MBLicence: CC BY 4.0

Spatio-temporal techniques for multi-dimensional imaging
Kallepalli, A. (Principal Investigator)
1/01/23 → 31/12/25
Project: Research Fellowship

Cite this

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abstract = "Fluorescence microscopy is a well-known technique for studying biological samples. However, on a global scale, microscopy techniques (beyond brightfield imaging) are inaccessible in some countries. Additionally, proprietary microscopy hardware is not modular and cannot be adapted for different architectures. In this research, we demonstrate a modular epifluorescence microscopy system and image analysis technique developed with the motivation of achieving high-resolution imaging using low-cost modular hardware. We compare the performance of the (1000 GBP) OpenFlexure-inspired epifluorescence ModMicro system to a proprietary epifluorescence microscope. With the epifluorescence ModMicro system, we imaged 'datacubes' of 0.54 x 0.40 x 0.005 mm (in 100 steps of 50 nm each) size. The image data is enhanced with a novel sharpening algorithm. Combined (light sources, microscope hardware and image sharpness enhancement algorithm), a complete system for epifluorescence microscopy that is globally accessible and cost-effective is presented.",

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KW - Fluorescence imaging

KW - microscopy

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KW - biophotonics

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Modular microscopy (ModMicro) solution for volumetric epifluorescence microscopic imaging

Abstract

Funding

Keywords

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Spatio-temporal techniques for multi-dimensional imaging

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