Abstract
| Language | English |
|---|---|
| Pages | 60-63 |
| Number of pages | 4 |
| Journal | Nanoscale Horizons |
| Issue number | 1 |
| Early online date | 20 Nov 2015 |
| DOIs | |
| Publication status | Published - 1 Jan 2016 |
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Keywords
- glyconanoparticles
- cholera toxin B-subunit
- mixed-monolayer glyconanoparticle
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Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced raman spectroscopy. / Simpson, Jonathan; Craig, Derek; Faulds, Karen; Graham, Duncan.
In: Nanoscale Horizons, No. 1, 01.01.2016, p. 60-63.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced raman spectroscopy
AU - Simpson, Jonathan
AU - Craig, Derek
AU - Faulds, Karen
AU - Graham, Duncan
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The same interactions that a pathogen uses for establishment in a host can be exploited in its detection. The carbohydrates comprising the intestinal cell surface GM1-ganglioside, are targeted by vibrio cholerae via the lectin, cholera toxin, to initiate infection. We report on the preparation of mixed-monolayer, carbohydrate-coated silver nanoparticles (glyconanoparticles) for the sensitive (56 ng/mL), low volume detection of cholera toxin B-subunit (CTB) in synthetic freshwater samples and in 5 minutes by surface enhanced Raman spectroscopy (SERS). The detection limit falls within the recommended detection range and matches WHO approved test limits. PEGylated galactose and sialic acid are added in a specific ratio to coat the particles in GM1-ganglioside mimics for interaction with CTB and display a synergic effect greater than either glycan alone. This demonstrates the first use of a mixed-monolayer glyconanoparticle which mimics the GM1 ligand, allowing selective interaction with CTB.
AB - The same interactions that a pathogen uses for establishment in a host can be exploited in its detection. The carbohydrates comprising the intestinal cell surface GM1-ganglioside, are targeted by vibrio cholerae via the lectin, cholera toxin, to initiate infection. We report on the preparation of mixed-monolayer, carbohydrate-coated silver nanoparticles (glyconanoparticles) for the sensitive (56 ng/mL), low volume detection of cholera toxin B-subunit (CTB) in synthetic freshwater samples and in 5 minutes by surface enhanced Raman spectroscopy (SERS). The detection limit falls within the recommended detection range and matches WHO approved test limits. PEGylated galactose and sialic acid are added in a specific ratio to coat the particles in GM1-ganglioside mimics for interaction with CTB and display a synergic effect greater than either glycan alone. This demonstrates the first use of a mixed-monolayer glyconanoparticle which mimics the GM1 ligand, allowing selective interaction with CTB.
KW - glyconanoparticles
KW - cholera toxin B-subunit
KW - mixed-monolayer glyconanoparticle
UR - http://pubs.rsc.org/en/content/articlelanding/2016/nh/c5nh00036j#!divAbstract
U2 - 10.1039/C5NH00036J
DO - 10.1039/C5NH00036J
M3 - Article
SP - 60
EP - 63
JO - Nanoscale Horizons
T2 - Nanoscale Horizons
JF - Nanoscale Horizons
SN - 2055-6764
IS - 1
ER -