Projects per year
Abstract
Mitochondrial pH (pHmito) is intimately related to mitochondrial function and aberrant values for pHmito are linked to several disease states. We report the design, synthesis and application of mitokyne 1 – the first small molecule pHmito sensor for Stimulated Raman Scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.
| Original language | English |
|---|---|
| Pages (from-to) | 12786-12792 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 93 |
| Issue number | 37 |
| Early online date | 10 Sept 2021 |
| DOIs | |
| Publication status | Published - 21 Sept 2021 |
Keywords
- mitokyne
- ratiometric
- raman probe
- mitochondrial pH
- Stimulated Raman scattering (SRS)
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Dive into the research topics of 'Mitokyne: a ratiometric raman probe for mitochondrial pH'. Together they form a unique fingerprint.Projects
- 2 Finished
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Nanoanalysis for Advanced Materials and Healthcare - EPSRC strategic equipment
Martin, R. (Principal Investigator), Edwards, P. (Co-investigator), Faulds, K. (Co-investigator), Florence, A. (Co-investigator), Graham, D. (Co-investigator), Sefcik, J. (Co-investigator), Ter Horst, J. (Co-investigator), Trager-Cowan, C. (Co-investigator), Uttamchandani, D. (Co-investigator) & Wark, A. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
8/11/15 → 7/11/19
Project: Research
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University Of Strathclyde - Equipment Account
Gachagan, A. (Principal Investigator), He, W. (Principal Investigator), Jaroszynski, D. (Principal Investigator), Martin, R. (Principal Investigator), McArthur, S. (Principal Investigator), McArthur, S. (Principal Investigator), Connolly, P. (Co-investigator), Edwards, P. (Co-investigator), Faulds, K. (Co-investigator), Florence, A. (Co-investigator), Graham, D. (Co-investigator), Leithead, B. (Co-investigator), Sefcik, J. (Co-investigator), Ter Horst, J. (Co-investigator), Trager-Cowan, C. (Co-investigator), Uttamchandani, D. (Co-investigator) & Wark, A. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/08/13 → 28/02/23
Project: Research