Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles

Alexandra Brito, Yousef M. Abul-Haija, Diana Soares Da Costa, Ramon Novoa-Carballal, Rui L. Reis, Rein V. Ulijn, Ricardo A. Pires, Iva Pashkuleva

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report the co-assembly of aromatic carbohydrate and dipeptide amphiphiles under physiological conditions as a strategy to generate minimalistic proteoglycan mimics. The resulting nanofibers present a structural, fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) core and a functional carbohydrate (Fmoc-glucosamine-6-sulfate or -phosphate) shell. The size, degree of bundling and mechanical properties of the assembled structures depend on the chemical nature of the carbohydrate amphiphile used. In cell culture medium, these nanofibers can further organize into supramolecular hydrogels. We demonstrate that, similar to proteoglycans, the assembled gels prolong the stability of growth factors and preserve the viability of cultured cells. Our results demonstrate that this approach can be applied to the design of extracellular matrix (ECM) substitutes for future regenerative therapies.

LanguageEnglish
Pages2385-2390
Number of pages6
JournalChemical Science
Volume10
Issue number8
Early online date21 Dec 2018
DOIs
Publication statusPublished - 28 Feb 2019

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Amphiphiles
Proteoglycans
Carbohydrates
Nanofibers
Peptides
Hydrogels
Dipeptides
Glucosamine
Cell culture
Sulfates
Culture Media
Intercellular Signaling Peptides and Proteins
Gels
Phosphates
Cells
Mechanical properties

Keywords

  • aromatic carbohydrate
  • supramolecular hydrogels
  • carbohydrate amphiphiles

Cite this

Brito, A., Abul-Haija, Y. M., Da Costa, D. S., Novoa-Carballal, R., Reis, R. L., Ulijn, R. V., ... Pashkuleva, I. (2019). Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. Chemical Science, 10(8), 2385-2390. https://doi.org/10.1039/C8SC04361B
Brito, Alexandra ; Abul-Haija, Yousef M. ; Da Costa, Diana Soares ; Novoa-Carballal, Ramon ; Reis, Rui L. ; Ulijn, Rein V. ; Pires, Ricardo A. ; Pashkuleva, Iva. / Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. In: Chemical Science. 2019 ; Vol. 10, No. 8. pp. 2385-2390.
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Brito, A, Abul-Haija, YM, Da Costa, DS, Novoa-Carballal, R, Reis, RL, Ulijn, RV, Pires, RA & Pashkuleva, I 2019, 'Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles' Chemical Science, vol. 10, no. 8, pp. 2385-2390. https://doi.org/10.1039/C8SC04361B

Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. / Brito, Alexandra; Abul-Haija, Yousef M.; Da Costa, Diana Soares; Novoa-Carballal, Ramon; Reis, Rui L.; Ulijn, Rein V.; Pires, Ricardo A.; Pashkuleva, Iva.

In: Chemical Science, Vol. 10, No. 8, 28.02.2019, p. 2385-2390.

Research output: Contribution to journalArticle

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AU - Brito, Alexandra

AU - Abul-Haija, Yousef M.

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AU - Novoa-Carballal, Ramon

AU - Reis, Rui L.

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