Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles

Alexandra Brito, Yousef M. Abul-Haija, Diana Soares Da Costa, Ramon Novoa-Carballal, Rui L. Reis, Rein V. Ulijn, Ricardo A. Pires, Iva Pashkuleva

Research output: Contribution to journalArticle

7 Citations (Scopus)
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Abstract

We report the co-assembly of aromatic carbohydrate and dipeptide amphiphiles under physiological conditions as a strategy to generate minimalistic proteoglycan mimics. The resulting nanofibers present a structural, fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) core and a functional carbohydrate (Fmoc-glucosamine-6-sulfate or -phosphate) shell. The size, degree of bundling and mechanical properties of the assembled structures depend on the chemical nature of the carbohydrate amphiphile used. In cell culture medium, these nanofibers can further organize into supramolecular hydrogels. We demonstrate that, similar to proteoglycans, the assembled gels prolong the stability of growth factors and preserve the viability of cultured cells. Our results demonstrate that this approach can be applied to the design of extracellular matrix (ECM) substitutes for future regenerative therapies.

Original languageEnglish
Pages (from-to)2385-2390
Number of pages6
JournalChemical Science
Volume10
Issue number8
Early online date21 Dec 2018
DOIs
Publication statusPublished - 28 Feb 2019

Keywords

  • aromatic carbohydrate
  • supramolecular hydrogels
  • carbohydrate amphiphiles

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    Brito, A., Abul-Haija, Y. M., Da Costa, D. S., Novoa-Carballal, R., Reis, R. L., Ulijn, R. V., ... Pashkuleva, I. (2019). Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. Chemical Science, 10(8), 2385-2390. https://doi.org/10.1039/C8SC04361B