Projects per year
Abstract
We present the development and performance characterisation of a novel structured illumination microscope (SIM) in which the grating pattern is generated using two optical beams controlled via 2 micro-electro-mechanical system (MEMS) three-axis scanning micromirrors. The implementation of MEMS micromirrors to accurately and repeatably control angular, radial and phase positioning delivers flexible control of the fluorescence excitation illumination, with achromatic beam delivery through the same optical path, reduced spatial footprint and cost-efficient integration being further benefits. Our SIM architecture enables the direct implementation of multi-color imaging in a compact and adaptable package. The two-dimensional SIM system approach is enabled by a pair of 2 mm aperture electrostatically actuated three-axis micromirrors having static angular tilt motion along the x- and y-axes and static piston motion along the z-axis. This allows precise angular, radial and phase positioning of two optical beams, generating a fully controllable spatial interference pattern at the focal plane by adjusting the positions of the beam in the back-aperture of a microscope objective. This MEMS-SIM system was applied to fluorescent bead samples and cell specimens, and was able to obtain a variable lateral resolution improvement between 1.3 and 1.8 times the diffraction limited resolution.
Original language | English |
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Pages (from-to) | 6443-6456 |
Number of pages | 14 |
Journal | Biomedical Optics Express |
Volume | 13 |
Issue number | 12 |
Early online date | 15 Nov 2022 |
DOIs | |
Publication status | Published - 1 Dec 2022 |
Keywords
- miniaturized structured illumination
- microscopy
- 3-axis MEMS micromirrors
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Doctoral Training Partnership 2020-2021 University of Strathclyde | Christopher, Jay
Bauer, R., Murray, P. & Christopher, J.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/20 → 1/04/24
Project: Research Studentship - Internally Allocated
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Doctoral Training Partnership 2018-19 University of Strathclyde | Donnachie, Mark
Bauer, R., Uttamchandani, D. & Donnachie, M.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/18 → 1/07/22
Project: Research Studentship - Internally Allocated
Datasets
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Data for: "Miniaturised structured illumination microscopy using two 3-axis MEMS micromirrors "
Bauer, R. (Supervisor), Tinning, P. W. (Creator), Christopher, J. (Contributor), Donnachie, M. (Contributor) & Uttamchandani, D. (Supervisor), University of Strathclyde, 11 Sep 2022
DOI: 10.15129/fb83f3fa-4e7f-4049-854a-9e3325cd9779
Dataset