Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system

Mairi E. Sandison; Michele Zagnoni; Mustafa Abu-Hantash; Hywel Morgan

doi:doi:10.1088/0960-1317/17/7/S17

Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system

Mairi E. Sandison, Michele Zagnoni, Mustafa Abu-Hantash, Hywel Morgan

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The use of spark assisted chemical engraving (SACE) to produce glass apertures that are suitable for the formation of artificial bilayer lipid membranes is described. Prior to use, the glass apertures were rendered hydrophobic by a silanization process and were then incorporated into a simple microfluidic device. Successful bilayer lipid membrane (BLM) formation and the subsequent acquisition of single-channel recordings are demonstrated. Due to the simplicity and rapidity of the SACE process, these glass apertures could be easily integrated into an all-glass microfluidic system for BLM formation.

Original language	English
Pages (from-to)	S189-S196
Number of pages	8
Journal	Journal of Micromechanics and Microengineering
Volume	17
Issue number	7
DOIs	https://doi.org/doi:10.1088/0960-1317/17/7/S17
Publication status	Published - 2 Jul 2007

Keywords

micromachining
glass apertures
artificial lipid bilayer
biological physics

Access to Document

doi:10.1088/0960-1317/17/7/S17

Cite this

@article{643d94faace84444a596be20640592eb,

title = "Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system",

abstract = "The use of spark assisted chemical engraving (SACE) to produce glass apertures that are suitable for the formation of artificial bilayer lipid membranes is described. Prior to use, the glass apertures were rendered hydrophobic by a silanization process and were then incorporated into a simple microfluidic device. Successful bilayer lipid membrane (BLM) formation and the subsequent acquisition of single-channel recordings are demonstrated. Due to the simplicity and rapidity of the SACE process, these glass apertures could be easily integrated into an all-glass microfluidic system for BLM formation.",

keywords = "micromachining, glass apertures, artificial lipid bilayer, biological physics",

author = "Sandison, {Mairi E.} and Michele Zagnoni and Mustafa Abu-Hantash and Hywel Morgan",

year = "2007",

month = jul,

day = "2",

doi = "doi:10.1088/0960-1317/17/7/S17",

language = "English",

volume = "17",

pages = "S189--S196 ",

journal = "Journal of Micromechanics and Microengineering",

issn = "0960-1317",

number = "7",

}

TY - JOUR

T1 - Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system

AU - Sandison, Mairi E.

AU - Zagnoni, Michele

AU - Abu-Hantash, Mustafa

AU - Morgan, Hywel

PY - 2007/7/2

Y1 - 2007/7/2

N2 - The use of spark assisted chemical engraving (SACE) to produce glass apertures that are suitable for the formation of artificial bilayer lipid membranes is described. Prior to use, the glass apertures were rendered hydrophobic by a silanization process and were then incorporated into a simple microfluidic device. Successful bilayer lipid membrane (BLM) formation and the subsequent acquisition of single-channel recordings are demonstrated. Due to the simplicity and rapidity of the SACE process, these glass apertures could be easily integrated into an all-glass microfluidic system for BLM formation.

AB - The use of spark assisted chemical engraving (SACE) to produce glass apertures that are suitable for the formation of artificial bilayer lipid membranes is described. Prior to use, the glass apertures were rendered hydrophobic by a silanization process and were then incorporated into a simple microfluidic device. Successful bilayer lipid membrane (BLM) formation and the subsequent acquisition of single-channel recordings are demonstrated. Due to the simplicity and rapidity of the SACE process, these glass apertures could be easily integrated into an all-glass microfluidic system for BLM formation.

KW - micromachining

KW - glass apertures

KW - artificial lipid bilayer

KW - biological physics

U2 - doi:10.1088/0960-1317/17/7/S17

DO - doi:10.1088/0960-1317/17/7/S17

M3 - Article

SN - 0960-1317

VL - 17

SP - S189-S196

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

IS - 7

ER -

Micromachined glass apertures for artificial lipid bilayer formation in a microfluidic system

Abstract

Keywords

Access to Document

Fingerprint

Cite this