Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

M. Jimenez*, H. Bridle

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also bacteria.

Original languageEnglish
Pages (from-to)8-11
Number of pages4
JournalJournal of Microbiological Methods
Volume126
Early online date30 Apr 2016
DOIs
Publication statusPublished - 1 Jul 2016

Funding

M.J. and H.B. would like to would like to acknowledge EU funding for the project “AQUAVALENS: protecting the health of Europeans by improving methods for the detection of pathogens in drinking water and water used in food preparation” and the EPSRC Impact Acceleration Award. H.B. would like to acknowledge The Royal Academy of Engineering/EPSRC for her research fellowship. M.J. and H.B. would like to acknowledge Epigem for the manufacturing part and Brian Miller for design considerations of inertial focussing devices in this type of application.

Keywords

  • Concentration
  • Cryptosporidium
  • Giardia
  • Microfluidics
  • Protozoa
  • Recovery rate
  • Sample processing
  • Waterborne disease

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