Abstract
We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also bacteria.
Original language | English |
---|---|
Pages (from-to) | 8-11 |
Number of pages | 4 |
Journal | Journal of Microbiological Methods |
Volume | 126 |
Early online date | 30 Apr 2016 |
DOIs | |
Publication status | Published - 1 Jul 2016 |
Funding
M.J. and H.B. would like to would like to acknowledge EU funding for the project “AQUAVALENS: protecting the health of Europeans by improving methods for the detection of pathogens in drinking water and water used in food preparation” and the EPSRC Impact Acceleration Award. H.B. would like to acknowledge The Royal Academy of Engineering/EPSRC for her research fellowship. M.J. and H.B. would like to acknowledge Epigem for the manufacturing part and Brian Miller for design considerations of inertial focussing devices in this type of application.
Keywords
- Concentration
- Cryptosporidium
- Giardia
- Microfluidics
- Protozoa
- Recovery rate
- Sample processing
- Waterborne disease