Microfluidic DNA amplification - a review

Yonghao Zhang, Pinar Ozdemir

Research output: Contribution to journalArticle

232 Citations (Scopus)
62 Downloads (Pure)

Abstract

The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the optimal design and operation of the device. In particular, we focus on continuous-flow microfluidic PCR on-chip, which can be readily implemented as an integrated function of a micro-total-analysis system. To overcome sample carryover contamination and surface adsorption associated with microfluidic PCR, microdroplet technology has recently been utilized to perform PCR in droplets, which can eliminate the synthesis of short chimeric products, shorten thermal-cycling time, and offers great potential for single DNA molecule and single-cell amplification. The work on chip-based PCR in droplets is highlighted.
Original languageEnglish
Pages (from-to)115-125
Number of pages10
JournalAnalytica Chimica Acta
Volume638
Issue number2
DOIs
Publication statusPublished - May 2009

Keywords

  • PCR
  • microfluidics
  • DNA amplification
  • microdroplet technology
  • Lab-on-a-chip
  • μTAS

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