Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug

Elisabeth Kastner, Varun Verma, Deborah Lowry, Yvonne Perrie

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

LanguageEnglish
Pages122-130
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume485
Issue number1-2
Early online date25 Feb 2015
DOIs
Publication statusPublished - 15 May 2015

Fingerprint

Microfluidics
Liposomes
Water
Pharmaceutical Preparations
Propofol
Solubility
Technology
Costs and Cost Analysis

Keywords

  • chemistry, pharmaceutical
  • cholesterol
  • kinetics
  • lab-on-a-chip devices
  • microfluidic analytical techniques
  • nanoparticles
  • particle size
  • phosphatidylcholines
  • propofol
  • solubility
  • solvents
  • technology, pharmaceutical
  • unilamellar liposomes

Cite this

@article{17882c282386442a9579f7a1d9a49fa4,
title = "Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug",
abstract = "Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol{\%}) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.",
keywords = "chemistry, pharmaceutical, cholesterol, kinetics, lab-on-a-chip devices, microfluidic analytical techniques, nanoparticles, particle size, phosphatidylcholines, propofol, solubility, solvents, technology, pharmaceutical, unilamellar liposomes",
author = "Elisabeth Kastner and Varun Verma and Deborah Lowry and Yvonne Perrie",
note = "Copyright {\circledC} 2015 Elsevier B.V. All rights reserved.",
year = "2015",
month = "5",
day = "15",
doi = "10.1016/j.ijpharm.2015.02.063",
language = "English",
volume = "485",
pages = "122--130",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
number = "1-2",

}

Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug. / Kastner, Elisabeth; Verma, Varun; Lowry, Deborah; Perrie, Yvonne.

In: International Journal of Pharmaceutics, Vol. 485, No. 1-2, 15.05.2015, p. 122-130.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microfluidic-controlled manufacture of liposomes for the solubilisation of a poorly water soluble drug

AU - Kastner, Elisabeth

AU - Verma, Varun

AU - Lowry, Deborah

AU - Perrie, Yvonne

N1 - Copyright © 2015 Elsevier B.V. All rights reserved.

PY - 2015/5/15

Y1 - 2015/5/15

N2 - Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

AB - Besides their well-described use as delivery systems for water-soluble drugs, liposomes have the ability to act as a solubilizing agent for drugs with low aqueous solubility. However, a key limitation in exploiting liposome technology is the availability of scalable, low-cost production methods for the preparation of liposomes. Here we describe a new method, using microfluidics, to prepare liposomal solubilising systems which can incorporate low solubility drugs (in this case propofol). The setup, based on a chaotic advection micromixer, showed high drug loading (41 mol%) of propofol as well as the ability to manufacture vesicles with at prescribed sizes (between 50 and 450 nm) in a high-throughput setting. Our results demonstrate the ability of merging liposome manufacturing and drug encapsulation in a single process step, leading to an overall reduced process time. These studies emphasise the flexibility and ease of applying lab-on-a-chip microfluidics for the solubilisation of poorly water-soluble drugs.

KW - chemistry, pharmaceutical

KW - cholesterol

KW - kinetics

KW - lab-on-a-chip devices

KW - microfluidic analytical techniques

KW - nanoparticles

KW - particle size

KW - phosphatidylcholines

KW - propofol

KW - solubility

KW - solvents

KW - technology, pharmaceutical

KW - unilamellar liposomes

UR - http://www.sciencedirect.com/science/article/pii/S0378517315001817

U2 - 10.1016/j.ijpharm.2015.02.063

DO - 10.1016/j.ijpharm.2015.02.063

M3 - Article

VL - 485

SP - 122

EP - 130

JO - International Journal of Pharmaceutics

T2 - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 1-2

ER -