MHz LED source for nanosecond fluorescence sensing

W.J. O'Hagan, M. McKenna, D.C. Sherrington, O.J. Rolinski, D.J.S. Birch

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

An inexpensive and portable pulsed light emitting diode (LED) source with variable repetition rate up to 10 MHz suitable for time-resolved fluorescence sensing, in medical, environmental and industrial applications is described. The pocket-sized battery-driven source is pulsed by signals from a simple logic circuit, giving measured optical pulse widths of similar to1.9 ns at 525 nm as recorded using, time-correlated single-photon counting. Pulses are presented for three LEDs with peak emissions at 525, 560 and 590 nm. The measurement performance is illustrated by the fluorescence lifetime and anisotropy decay of rhodamine 6G in various solvents and a fluorescence resonance energy transfer (FRET) sensor for detecting metal ions in water which uses a new measurand, namely the site distribution function rho(r) of metal ion acceptors accumulating in an anionic porous polymer with respect to the donor fluorophores. The rho(r) dependence reveals a minimum fluorophore-metal ion separation of similar to7 Angstrom which explains the selectivity of such FRET sensors in discriminating against other quenching mechanisms.
Original languageEnglish
Pages (from-to)84-91
Number of pages7
JournalMeasurement Science and Technology
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2002

Fingerprint

Diode
Fluorescence
Light emitting diodes
Metal ions
metal ions
Sensing
light emitting diodes
Fluorophores
Metals
Energy Transfer
resonance fluorescence
fluorescence
energy transfer
Fluorescence Lifetime
Sensor
logic circuits
Photon Counting
Performance Measurement
Logic circuits
sensors

Keywords

  • fluorescence lifetime sensing
  • pulsed LED
  • metal ions
  • fluorescence resonance energy transfer

Cite this

O'Hagan, W.J. ; McKenna, M. ; Sherrington, D.C. ; Rolinski, O.J. ; Birch, D.J.S. / MHz LED source for nanosecond fluorescence sensing. In: Measurement Science and Technology. 2002 ; Vol. 13, No. 1. pp. 84-91.
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MHz LED source for nanosecond fluorescence sensing. / O'Hagan, W.J.; McKenna, M.; Sherrington, D.C.; Rolinski, O.J.; Birch, D.J.S.

In: Measurement Science and Technology, Vol. 13, No. 1, 01.2002, p. 84-91.

Research output: Contribution to journalArticle

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T1 - MHz LED source for nanosecond fluorescence sensing

AU - O'Hagan, W.J.

AU - McKenna, M.

AU - Sherrington, D.C.

AU - Rolinski, O.J.

AU - Birch, D.J.S.

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N2 - An inexpensive and portable pulsed light emitting diode (LED) source with variable repetition rate up to 10 MHz suitable for time-resolved fluorescence sensing, in medical, environmental and industrial applications is described. The pocket-sized battery-driven source is pulsed by signals from a simple logic circuit, giving measured optical pulse widths of similar to1.9 ns at 525 nm as recorded using, time-correlated single-photon counting. Pulses are presented for three LEDs with peak emissions at 525, 560 and 590 nm. The measurement performance is illustrated by the fluorescence lifetime and anisotropy decay of rhodamine 6G in various solvents and a fluorescence resonance energy transfer (FRET) sensor for detecting metal ions in water which uses a new measurand, namely the site distribution function rho(r) of metal ion acceptors accumulating in an anionic porous polymer with respect to the donor fluorophores. The rho(r) dependence reveals a minimum fluorophore-metal ion separation of similar to7 Angstrom which explains the selectivity of such FRET sensors in discriminating against other quenching mechanisms.

AB - An inexpensive and portable pulsed light emitting diode (LED) source with variable repetition rate up to 10 MHz suitable for time-resolved fluorescence sensing, in medical, environmental and industrial applications is described. The pocket-sized battery-driven source is pulsed by signals from a simple logic circuit, giving measured optical pulse widths of similar to1.9 ns at 525 nm as recorded using, time-correlated single-photon counting. Pulses are presented for three LEDs with peak emissions at 525, 560 and 590 nm. The measurement performance is illustrated by the fluorescence lifetime and anisotropy decay of rhodamine 6G in various solvents and a fluorescence resonance energy transfer (FRET) sensor for detecting metal ions in water which uses a new measurand, namely the site distribution function rho(r) of metal ion acceptors accumulating in an anionic porous polymer with respect to the donor fluorophores. The rho(r) dependence reveals a minimum fluorophore-metal ion separation of similar to7 Angstrom which explains the selectivity of such FRET sensors in discriminating against other quenching mechanisms.

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