Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

Waqar M Ahmed, Paul Brinkman, Hans Weda, Hugo H Knobel, Yun Xu, Tamara M Nijsen, Roy Goodacre, Nicholas Rattray, Teunis J Vink, Marco Santonico, Giorgio Pennazza, Paulo Montuschi, Peter J Sterk, Stephen J Fowler

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Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.
Original languageEnglish
Number of pages14
JournalJournal of Breath Research
Issue number1
Early online date30 Oct 2018
Publication statusPublished - 30 Oct 2018


  • metabolomics
  • quality assurance
  • breath analysis
  • volatile organic compounds
  • asthma

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