Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project

Waqar M Ahmed, Paul Brinkman, Hans Weda, Hugo H Knobel, Yun Xu, Tamara M Nijsen, Roy Goodacre, Nicholas Rattray, Teunis J Vink, Marco Santonico, Giorgio Pennazza, Paulo Montuschi, Peter J Sterk, Stephen J Fowler

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.
LanguageEnglish
Number of pages14
JournalJournal of Breath Research
Volume13
Issue number1
Early online date30 Oct 2018
DOIs
Publication statusPublished - 30 Oct 2018

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Practice Guidelines
Asthma

Keywords

  • metabolomics
  • quality assurance
  • breath analysis
  • volatile organic compounds
  • asthma

Cite this

Ahmed, Waqar M ; Brinkman, Paul ; Weda, Hans ; Knobel, Hugo H ; Xu, Yun ; Nijsen, Tamara M ; Goodacre, Roy ; Rattray, Nicholas ; Vink, Teunis J ; Santonico, Marco ; Pennazza, Giorgio ; Montuschi, Paulo ; Sterk, Peter J ; Fowler, Stephen J. / Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project. In: Journal of Breath Research. 2018 ; Vol. 13, No. 1.
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abstract = "Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.",
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Ahmed, WM, Brinkman, P, Weda, H, Knobel, HH, Xu, Y, Nijsen, TM, Goodacre, R, Rattray, N, Vink, TJ, Santonico, M, Pennazza, G, Montuschi, P, Sterk, PJ & Fowler, SJ 2018, 'Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project' Journal of Breath Research, vol. 13, no. 1. https://doi.org/10.1088/1752-7163/aae557

Methodological considerations for large-scale breath analysis studies : lessons from the U-BIOPRED severe asthma project. / Ahmed, Waqar M; Brinkman, Paul; Weda, Hans; Knobel, Hugo H; Xu, Yun; Nijsen, Tamara M; Goodacre, Roy; Rattray, Nicholas; Vink, Teunis J; Santonico, Marco; Pennazza, Giorgio; Montuschi, Paulo; Sterk, Peter J; Fowler, Stephen J.

In: Journal of Breath Research, Vol. 13, No. 1, 30.10.2018.

Research output: Contribution to journalArticle

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AU - Santonico, Marco

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AU - Fowler, Stephen J

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