Metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain

A F Tawfike, R Tate, G Abbott, L Young, C Viegelmann, Marc Schumacher, Marc Diederich, R.A. Edrada-Ebel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. In this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate anticancer secondary metabolites from an endophytic Aspergillus aculeatus. The endophyte was isolated from the Egyptian medicinal plant Terminalia laxiflora and identified using molecular biological methods. Metabolomics and dereplication studies were accomplished by utilizing the MZmine software coupled with the universal Dictionary of Natural Products database. Metabolic profiling, with aid of multivariate data analysis, was performed at different stages of the growth curve to choose the optimised method suitable for up-scaling. The optimised culture method yielded a crude extract abundant with biologically-active secondary metabolites. Crude extracts were fractionated using different high-throughput chromatographic techniques. Purified compounds were identified by HRESI-MS, 1D and 2D-NMR. This study introduced a new method of dereplication utilising both high-resolution mass spectrometry and NMR spectroscopy. The metabolites were putatively identified by applying a chemotaxonomic filter. We also present a short review on the diverse chemistry of terrestrial endophytic strains of Aspergillus, which has become a part of our dereplication work and this will be of wide interest to those working in this field. This article is protected by copyright. All rights reserved.

LanguageEnglish
JournalChemistry and Biodiversity
Early online date3 Jul 2017
DOIs
Publication statusE-pub ahead of print - 3 Jul 2017

Fingerprint

Metabolomics
Aspergillus
Metabolites
Bioactivity
Complex Mixtures
Medicinal Plants
Agrochemicals
Mass Spectrometry
Terminalia
Glossaries
Endophytes
Biological Products
Fungi
Pharmaceutical Preparations
Drug products
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Throughput
Nuclear magnetic resonance
Magnetic Resonance Spectroscopy

Keywords

  • endophytic fungi
  • Aspergillus
  • dereplication
  • metabolomics

Cite this

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Metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain. / Tawfike, A F; Tate, R; Abbott, G; Young, L; Viegelmann, C; Schumacher, Marc; Diederich, Marc; Edrada-Ebel, R.A.

In: Chemistry and Biodiversity, 03.07.2017.

Research output: Contribution to journalArticle

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AU - Tate, R

AU - Abbott, G

AU - Young, L

AU - Viegelmann, C

AU - Schumacher, Marc

AU - Diederich, Marc

AU - Edrada-Ebel, R.A.

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N2 - Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. In this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate anticancer secondary metabolites from an endophytic Aspergillus aculeatus. The endophyte was isolated from the Egyptian medicinal plant Terminalia laxiflora and identified using molecular biological methods. Metabolomics and dereplication studies were accomplished by utilizing the MZmine software coupled with the universal Dictionary of Natural Products database. Metabolic profiling, with aid of multivariate data analysis, was performed at different stages of the growth curve to choose the optimised method suitable for up-scaling. The optimised culture method yielded a crude extract abundant with biologically-active secondary metabolites. Crude extracts were fractionated using different high-throughput chromatographic techniques. Purified compounds were identified by HRESI-MS, 1D and 2D-NMR. This study introduced a new method of dereplication utilising both high-resolution mass spectrometry and NMR spectroscopy. The metabolites were putatively identified by applying a chemotaxonomic filter. We also present a short review on the diverse chemistry of terrestrial endophytic strains of Aspergillus, which has become a part of our dereplication work and this will be of wide interest to those working in this field. This article is protected by copyright. All rights reserved.

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