TY - JOUR
T1 - Metabolomic strategies in dereplication for targeted cultivation and isolation of new bioactive secondary metabolites from fungal endophytes and marine microbial symbionts
AU - Cheng, Cheng
AU - Abdelmohsen, Usama Ramada
AU - Grkovic, Tanja
AU - Quinn, Ronald
AU - Hentschel, Ute
AU - Edrada-Ebel, Ruangelie
PY - 2013/8
Y1 - 2013/8
N2 - High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complimentary metabolomic tools to dereplicate chemical profiles of sponge-associated bacteria and fungal endophytes. The innovative strategy involved targeted cultivation and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) Analysis were used to analyze the HRFTMS and NMR data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites. Sponge-derived actinomycetes have become a rich source of new natural products with interesting pharmacological activities. New O-glycosylated angucyclines, were isolated from the broth culture of Actinokineospora sp. strain EG49 which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structure of the compounds were determined by 1D- and 2D-NMR techniques as well as high resolution tandem mass spectrometry.
AB - High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complimentary metabolomic tools to dereplicate chemical profiles of sponge-associated bacteria and fungal endophytes. The innovative strategy involved targeted cultivation and isolation of biologically active compounds. Principal Component (PCA), Hierarchical Clustering (HCA), and Orthogonal Partial Least Square-Discriminant (OPLS-DA) Analysis were used to analyze the HRFTMS and NMR data of culture extracts. The results of the statistical analysis identified and validated the best culture conditions and extraction procedure which optimized the isolation of novel bioactive metabolites. Sponge-derived actinomycetes have become a rich source of new natural products with interesting pharmacological activities. New O-glycosylated angucyclines, were isolated from the broth culture of Actinokineospora sp. strain EG49 which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structure of the compounds were determined by 1D- and 2D-NMR techniques as well as high resolution tandem mass spectrometry.
KW - high resolution Fourier transform mass spectrometry
KW - HRFTMS
KW - nuclear magnetic resonance spectroscopy
KW - NMR
KW - sponge-associated bacteria
KW - fungal endophytes
KW - bioactive metabolites
KW - sponge-derived actinomycetes
UR - http://www.thieme.com/
U2 - 10.1055/s-0033-1351855
DO - 10.1055/s-0033-1351855
M3 - Conference Contribution
SN - 0032-0943
VL - 79
JO - Planta Medica
JF - Planta Medica
IS - 13
M1 - SL29
T2 - 61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research
Y2 - 1 September 2013 through 5 September 2013
ER -