Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry

M.A. Kamleh, D.G. Watson, Y. Hobani, J.A.T. Dow

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy (ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected.
Original languageEnglish
Pages (from-to)2916-2922
Number of pages6
JournalFEBS Letters
Volume582
Issue number19
DOIs
Publication statusPublished - 20 Aug 2008

Fingerprint

Allantoin
Glycerophospholipids
Hypoxanthine
Xanthine
Metabolomics
Xanthine Oxidase
Liquid chromatography
Mass spectrometers
Fourier Analysis
Chromatography
Uric Acid
Metabolism
Tryptophan
Liquid Chromatography
Drosophila
Mass spectrometry
Arginine
Mass Spectrometry
Fourier transforms
Oxidoreductases

Keywords

  • Metabolomics
  • Fourier transform mass spectrometry
  • Drosophila

Cite this

Kamleh, M.A. ; Watson, D.G. ; Hobani, Y. ; Dow, J.A.T. / Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry. In: FEBS Letters. 2008 ; Vol. 582, No. 19. pp. 2916-2922.
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Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry. / Kamleh, M.A.; Watson, D.G.; Hobani, Y.; Dow, J.A.T.

In: FEBS Letters, Vol. 582, No. 19, 20.08.2008, p. 2916-2922.

Research output: Contribution to journalArticle

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