Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry

M.A. Kamleh, D.G. Watson, Y. Hobani, J.A.T. Dow

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy (ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected.
Original languageEnglish
Pages (from-to)2916-2922
Number of pages6
JournalFEBS Letters
Volume582
Issue number19
DOIs
Publication statusPublished - 20 Aug 2008

Keywords

  • Metabolomics
  • Fourier transform mass spectrometry
  • Drosophila

Fingerprint Dive into the research topics of 'Metabolomic profiling of Drosophila using liquid chromatography Fourier transform mass spectrometry'. Together they form a unique fingerprint.

Cite this