Research Output per year
Streptomyces sp. SM8 revealed the presence of multiple secondary metabolism gene clusters, including a gene cluster for the biosynthesis of the antifungal antimycin family of compounds. The antimycin gene cluster of Streptomyces sp. SM8 was inactivated by disruption of the antimycin biosynthesis gene ant C. Extracts from this mutant strain showed loss of antimycin production and significantly less antifungal activity than the wild-type strain. Three butenolides, 4,10-dihydroxy-10-methyl-dodec-2-en-1,4-olide (1),4,11-dihydroxy-10-methyl-dodec-2-en-1,4-olide (2), and 4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide(3) that had previously been reported from marine Streptomyces species were also isolated from SM8. Comparison of the extracts of Streptomyces strain SM8 and its host sponge, H. simulans, using LC-Orbitrap revealed the presence of metabolites common to both extracts, providing direct evidence linking sponge metabolites to a specific microbial symbiont.
- haliclona simulans
Research output: Contribution to journal › Literature review
Metabolomics as a tool in the identification and production of new antibiotics from sponges and endosymbiotic bacteriaViegelmann, C. V., Hoe Leong, C., Clements, C., Abdelmohsen, U. R., Hentschel, U., Margassery, L. M., Kennedy, J., Perrocco, D., Dobson, A. D. W. & Edrada-Ebel, R., Feb 2013.
Research output: Contribution to conference › Poster