Metabolomic profiling and genomic study of a marine sponge-associated streptomyces sp

Christina Victoria Viegelmann, Lekha Menon Margassery, Jonathan Kennedy, Tong Zhang, Ciarán O’Brien, Fergal O’Gara, John P. Morrissey, Alan D. W. Dobson, Ruangelie Edrada-Ebel

Research output: Contribution to journalArticle

32 Citations (Scopus)
67 Downloads (Pure)

Abstract

T Metabolomics and genomics are two complementary platforms for analyzing an organism as they provide information on the phenotype and genotype, respectively. These two techniques were applied in the dereplication and identification of bioactive compounds from a Streptomyces sp. (SM8) isolated from the sponge Haliclona simulans from Irish waters. Streptomyces strain SM8 extracts showed antibacterial and antifungal activity. NMR analysis of the active fractions proved that hydroxylated saturated fatty acids were the major components present in the antibacterial fractions. Antimycin compounds were initially putatively identified in the antifungal fractions using LC-Orbitrap. Their presence was later confirmed by comparison to a standard. Genomic analysis of
Streptomyces sp. SM8 revealed the presence of multiple secondary metabolism gene clusters, including a gene cluster for the biosynthesis of the antifungal antimycin family of compounds. The antimycin gene cluster of Streptomyces sp. SM8 was inactivated by disruption of the antimycin biosynthesis gene ant C. Extracts from this mutant strain showed loss of antimycin production and significantly less antifungal activity than the wild-type strain. Three butenolides, 4,10-dihydroxy-10-methyl-dodec-2-en-1,4-olide (1),4,11-dihydroxy-10-methyl-dodec-2-en-1,4-olide (2), and 4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide(3) that had previously been reported from marine Streptomyces species were also isolated from SM8. Comparison of the extracts of Streptomyces strain SM8 and its host sponge, H. simulans, using LC-Orbitrap revealed the presence of metabolites common to both extracts, providing direct evidence linking sponge metabolites to a specific microbial symbiont.
Original languageEnglish
Pages (from-to)3323-3351
Number of pages29
JournalMarine Drugs
Volume12
Issue number6
DOIs
Publication statusPublished - 2 Jun 2014

Keywords

  • streptomyces
  • haliclona simulans
  • metabolomics
  • antimycin
  • antifungal
  • butenolide

Research Output

  • 32 Citations
  • 2 Poster
  • 1 Literature review

The re-emergence of natural products for drug discovery in the genomics era

Harvey, A., Edrada-Ebel, R. & Quinn, R. J., 23 Jan 2015, In : Nature Reviews Drug Discovery . 14, p. 111–129 19 p.

Research output: Contribution to journalLiterature review

Open Access
File
1021 Citations (Scopus)
568 Downloads (Pure)

Metabolomics as a tool in the identification and production of new antibiotics from sponges and endosymbiotic bacteria

Viegelmann, C. V., Hoe Leong, C., Clements, C., Abdelmohsen, U. R., Hentschel, U., Margassery, L. M., Kennedy, J., Perrocco, D., Dobson, A. D. W. & Edrada-Ebel, R., Feb 2013.

Research output: Contribution to conferencePoster

Genomic mining of bioactive marine Streptomyces

Kennedy, J., Margassery, L. M., O'Brien, C., O'Neill, I., Edrada-Ebel, R., Viegelmann, C. V., O’Gara, F., Morrissey, J. P. & Dobson, A., Sep 2012.

Research output: Contribution to conferencePoster

Activities

  • 1 Hosting an academic visitor

Burkhardt Flemer

Ruangelie Edrada-Ebel (Host)

1 Aug 201121 Aug 2011

Activity: Hosting a visitor typesHosting an academic visitor

Cite this

Viegelmann, C. V., Margassery, L. M., Kennedy, J., Zhang, T., O’Brien, C., O’Gara, F., Morrissey, J. P., Dobson, A. D. W., & Edrada-Ebel, R. (2014). Metabolomic profiling and genomic study of a marine sponge-associated streptomyces sp. Marine Drugs, 12(6), 3323-3351. https://doi.org/10.3390/md12063323