Metabolism of quercetin and kaempferol by rat hepatocytes and identification of flavonoid glycosides in plasma

E.D.J. Oliveira, D.G. Watson, M.H. Grant

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The metabolism of the flavonoids quercetin and kaempferol by rat hepatocytes was investigated using liquid chromatography coupled with electrospray mass spectrometry (LC-ESI MS). Quercetin and kaempferol were extensively metabolized (98.8 +/- 0.1% and 81.0 +/- 5.1% respectively, n = 4), with four glucuronides of quercetin and two of kaempferol being detected after incubation. 2. The glucuronides of quercetin and kaempferol formed upon incubation with rat hepatocytes were identified as the same ones formed after incubation with the UDP-glucuronosyltransferase isoform UGT1A9. 3. In addition, plasma samples from human volunteers taken after consumption of capsules of Ginkgo biloba, a plant rich in flavonoid glycosides, were analysed by LC-MS for the presence of flavonoid glucuronides and flavonoid glycosides. Reported is evidence for the presence of flavonoid glycosides in samples of plasma. 4. The results suggest that UGT1A9 is a key UDP-glucuronosyltransferase isoform for the metabolism of flavonoids, and that absorption of intact flavonoid glycosides is possible.
LanguageEnglish
Pages279-287
Number of pages9
JournalXenobiotica
Volume32
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Quercetin
Glycosides
Flavonoids
Metabolism
Rats
Hepatocytes
Plasmas
Glucuronides
Glucuronosyltransferase
Protein Isoforms
Ginkgo biloba
Electrospray Ionization Mass Spectrometry
Liquid chromatography
kaempferol
Liquid Chromatography
Capsules
Mass spectrometry
Volunteers

Keywords

  • quercetin
  • kaempferol
  • rat hepatocytes
  • plasma
  • flavonoid glycosides

Cite this

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abstract = "The metabolism of the flavonoids quercetin and kaempferol by rat hepatocytes was investigated using liquid chromatography coupled with electrospray mass spectrometry (LC-ESI MS). Quercetin and kaempferol were extensively metabolized (98.8 +/- 0.1{\%} and 81.0 +/- 5.1{\%} respectively, n = 4), with four glucuronides of quercetin and two of kaempferol being detected after incubation. 2. The glucuronides of quercetin and kaempferol formed upon incubation with rat hepatocytes were identified as the same ones formed after incubation with the UDP-glucuronosyltransferase isoform UGT1A9. 3. In addition, plasma samples from human volunteers taken after consumption of capsules of Ginkgo biloba, a plant rich in flavonoid glycosides, were analysed by LC-MS for the presence of flavonoid glucuronides and flavonoid glycosides. Reported is evidence for the presence of flavonoid glycosides in samples of plasma. 4. The results suggest that UGT1A9 is a key UDP-glucuronosyltransferase isoform for the metabolism of flavonoids, and that absorption of intact flavonoid glycosides is possible.",
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Metabolism of quercetin and kaempferol by rat hepatocytes and identification of flavonoid glycosides in plasma. / Oliveira, E.D.J.; Watson, D.G.; Grant, M.H.

In: Xenobiotica, Vol. 32, No. 4, 2002, p. 279-287.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Watson, D.G.

AU - Grant, M.H.

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AB - The metabolism of the flavonoids quercetin and kaempferol by rat hepatocytes was investigated using liquid chromatography coupled with electrospray mass spectrometry (LC-ESI MS). Quercetin and kaempferol were extensively metabolized (98.8 +/- 0.1% and 81.0 +/- 5.1% respectively, n = 4), with four glucuronides of quercetin and two of kaempferol being detected after incubation. 2. The glucuronides of quercetin and kaempferol formed upon incubation with rat hepatocytes were identified as the same ones formed after incubation with the UDP-glucuronosyltransferase isoform UGT1A9. 3. In addition, plasma samples from human volunteers taken after consumption of capsules of Ginkgo biloba, a plant rich in flavonoid glycosides, were analysed by LC-MS for the presence of flavonoid glucuronides and flavonoid glycosides. Reported is evidence for the presence of flavonoid glycosides in samples of plasma. 4. The results suggest that UGT1A9 is a key UDP-glucuronosyltransferase isoform for the metabolism of flavonoids, and that absorption of intact flavonoid glycosides is possible.

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