Mechanisms and dynamics of protein clustering on a solid surface

P.A. Mulheran, D. Pellenc, R.A. Bennett, R.J. Green, M. Sperrin

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is presented. In situ atomic force microscopy images are quantitatively compared to Monte Carlo simulations using cluster statistics to differentiate various models.We study lysozyme adsorption on mica as a model system and find that all surface-supported clusters are mobile, not just the monomers, with diffusion constant inversely related to cluster size. The surface monomer diffusion constant is measured to be D-1 similar to 9x10(-16)cm(2) s(-1), such a low value being difficult to measure using other techniques.
LanguageEnglish
Article number068102
Number of pages4
JournalPhysical Review Letters
Volume100
Issue number6
DOIs
Publication statusPublished - 12 Feb 2008

Fingerprint

solid surfaces
proteins
monomers
lysozyme
mica
statistics
atomic force microscopy
methodology
adsorption
simulation

Keywords

  • protein clustering
  • mechanisms
  • dynamics
  • protein

Cite this

Mulheran, P.A. ; Pellenc, D. ; Bennett, R.A. ; Green, R.J. ; Sperrin, M. / Mechanisms and dynamics of protein clustering on a solid surface. In: Physical Review Letters. 2008 ; Vol. 100, No. 6.
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Mechanisms and dynamics of protein clustering on a solid surface. / Mulheran, P.A.; Pellenc, D.; Bennett, R.A.; Green, R.J.; Sperrin, M.

In: Physical Review Letters, Vol. 100, No. 6, 068102 , 12.02.2008.

Research output: Contribution to journalArticle

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