Mechanisms and dynamics of protein clustering on a solid surface

P.A. Mulheran, D. Pellenc, R.A. Bennett, R.J. Green, M. Sperrin

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41 Citations (Scopus)

Abstract

A methodology for discovering the mechanisms and dynamics of protein clustering on solid surfaces is presented. In situ atomic force microscopy images are quantitatively compared to Monte Carlo simulations using cluster statistics to differentiate various models.We study lysozyme adsorption on mica as a model system and find that all surface-supported clusters are mobile, not just the monomers, with diffusion constant inversely related to cluster size. The surface monomer diffusion constant is measured to be D-1 similar to 9x10(-16)cm(2) s(-1), such a low value being difficult to measure using other techniques.
Original languageEnglish
Article number068102
Number of pages4
JournalPhysical Review Letters
Volume100
Issue number6
DOIs
Publication statusPublished - 12 Feb 2008

Keywords

  • protein clustering
  • mechanisms
  • dynamics
  • protein

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