Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy

G.M. Gibson, J. Leach, S. Keen, A.J. Wright, M.J. Padgett

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

We assess the performance of a CMOS camera for the measurement of particle position within optical tweezers and the associated autocorrelation function and power spectrum. Measurement of the displacement of the particle from the trap center can also be related to the applied force. By considering the Allan variance of these measurements, we show that such cameras are capable of reaching the thermal limits of nanometer and femtonewton accuracies, and hence are suitable for many of the applications that traditionally use quadrant photodiodes. As an example of a multi-particle measurement we show the hydrodynamic coupling between two particles.
LanguageEnglish
Pages14561-14570
Number of pages10
JournalOptics Express
Volume16
Issue number19
DOIs
Publication statusPublished - 2 Sep 2008

Fingerprint

high speed
microscopy
cameras
quadrants
autocorrelation
photodiodes
power spectra
CMOS
hydrodynamics
traps

Keywords

  • laser trapping
  • optical confinement
  • optical manipulation
  • optical tweezers

Cite this

Gibson, G.M. ; Leach, J. ; Keen, S. ; Wright, A.J. ; Padgett, M.J. / Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy. In: Optics Express. 2008 ; Vol. 16, No. 19. pp. 14561-14570.
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Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy. / Gibson, G.M.; Leach, J.; Keen, S.; Wright, A.J.; Padgett, M.J.

In: Optics Express, Vol. 16, No. 19, 02.09.2008, p. 14561-14570.

Research output: Contribution to journalArticle

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