Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy

Katrin Adamczyk, Marco Candelaresi, Kirsty Robb, Andrea Gumiero, Martin A. Walsh, Anthony W. Parker, Paul A. Hoskisson, Nicholas P. Tucker, Neil T. Hunt

Research output: Contribution to journalLiterature review

20 Citations (Scopus)

Abstract

Recent advances in the methodology and application of ultrafast two-dimensional infrared (2D-IR) spectroscopy to biomolecular systems are reviewed. A description of the 2D-IR technique and the molecular contributions to the observed spectra are presented followed by a discussion of recent literature relating to the use of 2D-IR and associated approaches for measuring protein dynamics. In particular, these include the use of diatomic ligand groups for measuring haem protein dynamics, isotopic labelling strategies and the use of vibrational probe groups. The final section reports on the current state of the art regarding the use of 2D-IR methods to provide insights into biological reaction mechanisms.

LanguageEnglish
Article number062001
Number of pages16
JournalMeasurement Science and Technology
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Infrared Spectroscopy
Infrared spectroscopy
infrared spectroscopy
isotopic labeling
proteins
Proteins
Protein
Reaction Mechanism
Labeling
Spectroscopy
Infrared
Probe
Ligands
methodology
ligands
Methodology
probes
spectroscopy
Strategy

Keywords

  • medical physics
  • instrumentation
  • measurement
  • proteins

Cite this

Adamczyk, K., Candelaresi, M., Robb, K., Gumiero, A., Walsh, M. A., Parker, A. W., ... Hunt, N. T. (2012). Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy. Measurement Science and Technology , 23(6), [062001]. https://doi.org/10.1088/0957-0233/23/6/062001
Adamczyk, Katrin ; Candelaresi, Marco ; Robb, Kirsty ; Gumiero, Andrea ; Walsh, Martin A. ; Parker, Anthony W. ; Hoskisson, Paul A. ; Tucker, Nicholas P. ; Hunt, Neil T. / Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy. In: Measurement Science and Technology . 2012 ; Vol. 23, No. 6.
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Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy. / Adamczyk, Katrin; Candelaresi, Marco; Robb, Kirsty; Gumiero, Andrea; Walsh, Martin A.; Parker, Anthony W.; Hoskisson, Paul A.; Tucker, Nicholas P.; Hunt, Neil T.

In: Measurement Science and Technology , Vol. 23, No. 6, 062001, 06.2012.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy

AU - Adamczyk, Katrin

AU - Candelaresi, Marco

AU - Robb, Kirsty

AU - Gumiero, Andrea

AU - Walsh, Martin A.

AU - Parker, Anthony W.

AU - Hoskisson, Paul A.

AU - Tucker, Nicholas P.

AU - Hunt, Neil T.

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AB - Recent advances in the methodology and application of ultrafast two-dimensional infrared (2D-IR) spectroscopy to biomolecular systems are reviewed. A description of the 2D-IR technique and the molecular contributions to the observed spectra are presented followed by a discussion of recent literature relating to the use of 2D-IR and associated approaches for measuring protein dynamics. In particular, these include the use of diatomic ligand groups for measuring haem protein dynamics, isotopic labelling strategies and the use of vibrational probe groups. The final section reports on the current state of the art regarding the use of 2D-IR methods to provide insights into biological reaction mechanisms.

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KW - instrumentation

KW - measurement

KW - proteins

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