Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy

Katrin Adamczyk, Marco Candelaresi, Kirsty Robb, Andrea Gumiero, Martin A. Walsh, Anthony W. Parker, Paul A. Hoskisson, Nicholas P. Tucker, Neil T. Hunt

Research output: Contribution to journalLiterature reviewpeer-review

30 Citations (Scopus)


Recent advances in the methodology and application of ultrafast two-dimensional infrared (2D-IR) spectroscopy to biomolecular systems are reviewed. A description of the 2D-IR technique and the molecular contributions to the observed spectra are presented followed by a discussion of recent literature relating to the use of 2D-IR and associated approaches for measuring protein dynamics. In particular, these include the use of diatomic ligand groups for measuring haem protein dynamics, isotopic labelling strategies and the use of vibrational probe groups. The final section reports on the current state of the art regarding the use of 2D-IR methods to provide insights into biological reaction mechanisms.

Original languageEnglish
Article number062001
Number of pages16
JournalMeasurement Science and Technology
Issue number6
Publication statusPublished - Jun 2012


  • medical physics
  • instrumentation
  • measurement
  • proteins


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