TY - JOUR
T1 - Maximization of selectivity in reversed-phase liquid chromatographic method development strategies
AU - Euerby, Melvin R.
AU - Fever, Mark
AU - Hulse, Jennifer
AU - James, Matthew
AU - Petersson, Patrik
AU - Pipe, Colin
PY - 2016/1/30
Y1 - 2016/1/30
N2 - The selectivity of different combinations of organic modifiers, pH, and types of reversed-phase liquid chromatography (LC) materials has been characterized using Tanaka column characterization, linear solvent energy relationships (LSER), and selectivity correlations. The three characterization techniques highlighted the potential complementary selectivity of these phases and conditions as well as the type and dominancy of some of the retention mechanisms involved. Subsequently, selectivity differences were proven to be valid in the practical separation of acids, bases, and neutral analytes. This paper aims to assist chromatographers in producing highly efficiency method development strategies for reversed-phase LC separations in a relatively short time frame.
AB - The selectivity of different combinations of organic modifiers, pH, and types of reversed-phase liquid chromatography (LC) materials has been characterized using Tanaka column characterization, linear solvent energy relationships (LSER), and selectivity correlations. The three characterization techniques highlighted the potential complementary selectivity of these phases and conditions as well as the type and dominancy of some of the retention mechanisms involved. Subsequently, selectivity differences were proven to be valid in the practical separation of acids, bases, and neutral analytes. This paper aims to assist chromatographers in producing highly efficiency method development strategies for reversed-phase LC separations in a relatively short time frame.
KW - chromatography
KW - liquid chromatography
KW - linear solvent energy relationships
KW - reversed-phase
UR - http://www.scopus.com/inward/record.url?scp=84955289449&partnerID=8YFLogxK
UR - http://www.chromatographyonline.com/sourceissues
UR - https://www.chromatographyonline.com/view/maximization-selectivity-reversed-phase-liquid-chromatographic-method-development-strategies-0
M3 - Article
AN - SCOPUS:84955289449
SN - 1471-6577
VL - 29
SP - 8
EP - 21
JO - LC-GC Europe
JF - LC-GC Europe
IS - 1
ER -