Abstract
Human foetal skeletal muscle was grown in explant cultures and the development of myotubes was monitored morphologically and by the development of membrane potential and acetylcholine sensitivity. Migration of uninucleate cells from the explants occurred in the first day of culture and formation of multinucleated myotubes took place between 7 to 10 days. Early myotubes were variable in appearance, being either flat and nonrefractile or narrow and cylindrical. Some cross-striated cells were observed. Resting membrane potentials were around -25 mV and there was great variation in myotube sensitivity to acetylcholine. After about 6 weeks in culture most myotubes were of the refractile type. Many had hypolemmal nuclei and were cross-striated; some contracted spontaneously. All myotubes had developed high sensitivity to acetylcholine although there had been no increase in resting membrane potential.
| Language | English |
|---|---|
| Pages | 115-122 |
| Number of pages | 8 |
| Journal | Journal of the Neurological Sciences |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 1979 |
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Keywords
- acetylcholine
- fetus
- in vitro study
- muscle
- muscle fiber membrane potential
- normal human
- theoretical study
- cell differentiation
- dose-response relationship, drug
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Maturation of human skeletal muscle fibres in explant tissue culture. / Harvey, A. L.; Robertson, J. G. ; Witkowski, J. A.
In: Journal of the Neurological Sciences, Vol. 41, No. 1, 01.01.1979, p. 115-122.Research output: Contribution to journal › Article
TY - JOUR
T1 - Maturation of human skeletal muscle fibres in explant tissue culture
AU - Harvey, A. L.
AU - Robertson, J. G.
AU - Witkowski, J. A.
PY - 1979/1/1
Y1 - 1979/1/1
N2 - Human foetal skeletal muscle was grown in explant cultures and the development of myotubes was monitored morphologically and by the development of membrane potential and acetylcholine sensitivity. Migration of uninucleate cells from the explants occurred in the first day of culture and formation of multinucleated myotubes took place between 7 to 10 days. Early myotubes were variable in appearance, being either flat and nonrefractile or narrow and cylindrical. Some cross-striated cells were observed. Resting membrane potentials were around -25 mV and there was great variation in myotube sensitivity to acetylcholine. After about 6 weeks in culture most myotubes were of the refractile type. Many had hypolemmal nuclei and were cross-striated; some contracted spontaneously. All myotubes had developed high sensitivity to acetylcholine although there had been no increase in resting membrane potential.
AB - Human foetal skeletal muscle was grown in explant cultures and the development of myotubes was monitored morphologically and by the development of membrane potential and acetylcholine sensitivity. Migration of uninucleate cells from the explants occurred in the first day of culture and formation of multinucleated myotubes took place between 7 to 10 days. Early myotubes were variable in appearance, being either flat and nonrefractile or narrow and cylindrical. Some cross-striated cells were observed. Resting membrane potentials were around -25 mV and there was great variation in myotube sensitivity to acetylcholine. After about 6 weeks in culture most myotubes were of the refractile type. Many had hypolemmal nuclei and were cross-striated; some contracted spontaneously. All myotubes had developed high sensitivity to acetylcholine although there had been no increase in resting membrane potential.
KW - acetylcholine
KW - fetus
KW - in vitro study
KW - muscle
KW - muscle fiber membrane potential
KW - normal human
KW - theoretical study
KW - cell differentiation
KW - dose-response relationship, drug
UR - http://www.scopus.com/inward/record.url?scp=0018426181&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(79)90145-X
DO - 10.1016/0022-510X(79)90145-X
M3 - Article
VL - 41
SP - 115
EP - 122
JO - Journal of the Neurological Sciences
T2 - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 1
ER -