Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs)

F Philipp Seib, Marina Prewitz, Carsten Werner, Martin Bornhäuser

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically and biophysically distinct. Here we use well-defined in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs in vivo.
Original languageEnglish
Pages (from-to)663-667
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume389
Issue number4
DOIs
Publication statusPublished - 27 Nov 2009

Fingerprint

Elasticity
Mesenchymal Stromal Cells
Bone
Bone Marrow
Substrates
Hydrogel
Interleukin-8
Muscle
Brain
Intercellular Signaling Peptides and Proteins
Tissue
Cytokines
Proteins
Muscles
Therapeutics

Keywords

  • mesenchymal stem cells
  • growth factors
  • chemokine
  • compliance
  • stiffness
  • glycosan extracel
  • angiogenesis

Cite this

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Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (MSCs). / Seib, F Philipp; Prewitz, Marina; Werner, Carsten; Bornhäuser, Martin.

In: Biochemical and Biophysical Research Communications, Vol. 389, No. 4, 27.11.2009, p. 663-667.

Research output: Contribution to journalArticle

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AB - The therapeutic efficacy of multipotent mesenchymal stromal cells (MSCs) is attributed to particular MSC-derived cytokines and growth factors. As MSCs are applied locally to target organs or home there after systemic administration, they experience diverse microenvironments that are biochemically and biophysically distinct. Here we use well-defined in vitro conditions to study the impact of substrate elasticity on MSC-derived trophic factors. By varying hydrogel compliance, the elasticity of brain and muscle tissue was mimicked. We screened >90 secreted factors at the protein level, finding a diverse elasticity-dependent expression pattern. In particular, IL-8 was up-regulated as much as 90-fold in MSCs cultured for 2days on hard substrates, whereas levels were consistently low on soft substrates. In summary, we show substrate elasticity directly affects MSC paracrine expression, a relevant finding for therapies administering MSCs in vivo.

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