Mast cell subsets and their functional modulation by the Acanthocheilonema viteae product ES-62

Dimity Ball, Hwee Kee Tay, Kara Bell, Michelle Coates, Lamyaa Al-Riyami, Justyna Rzepecka, William Harnett, Margaret Harnett

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

ES-62, an immunomodulator secreted by filarial nematodes, exhibits therapeutic potential in mouse models of allergic inflammation, at least in part by inducing the desensitisation of Fc휀RI-mediated mast cell responses. However, in addition to their pathogenic roles in allergic and autoimmune diseases, mast cells are important in fighting infection, wound healing, and resolving inflammation, reflecting that mast cells exhibit a phenotypic and functional plasticity. We have therefore characterised the differential functional responses to antigen (via Fc휀RI) and LPS and their modulation by ES-62 of the mature peritoneal-derived mast cells (PDMC; serosal) and those of the connective tissue-like mast cells (CTMC) and themucosal-likemast cells derived from
bone marrow progenitors (BMMC) as a first step to produce disease tissue-targeted therapeutics based on ES-62 action. All three mast cell populations were rendered hyporesponsive by ES-62 and whilst the mechanisms underlying such desensitisation have not been fully delineated, they reflect a downregulation of calcium and PKC훼 signalling. ES-62 also downregulatedMyD88 and PKC훿 in mucosal-type BMMC but not PDMC, the additional signals targeted in mucosal-type BMMC likely reflecting that these cells respond to antigen and LPS by degranulation and cytokine secretion whereas PDMC predominantly respond in a degranulationbased manner.
LanguageEnglish
Article number961268
Number of pages13
JournalJournal of Parasitology Research
Volume2013
DOIs
Publication statusAccepted/In press - 2013

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Acanthocheilonema
Mast Cells
Inflammation
Antigens
Calcium Signaling
Immunologic Factors
Connective Tissue
Wound Healing
Autoimmune Diseases
Down-Regulation
Bone Marrow
Cytokines
Therapeutics
Infection

Keywords

  • ES-62
  • mast cell
  • immunomodulation

Cite this

Ball, D., Tay, H. K., Bell, K., Coates, M., Al-Riyami, L., Rzepecka, J., ... Harnett, M. (Accepted/In press). Mast cell subsets and their functional modulation by the Acanthocheilonema viteae product ES-62. Journal of Parasitology Research, 2013, [961268]. https://doi.org/10.1155/2013/961268
Ball, Dimity ; Tay, Hwee Kee ; Bell, Kara ; Coates, Michelle ; Al-Riyami, Lamyaa ; Rzepecka, Justyna ; Harnett, William ; Harnett, Margaret . / Mast cell subsets and their functional modulation by the Acanthocheilonema viteae product ES-62. In: Journal of Parasitology Research. 2013 ; Vol. 2013.
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Mast cell subsets and their functional modulation by the Acanthocheilonema viteae product ES-62. / Ball, Dimity; Tay, Hwee Kee; Bell, Kara; Coates, Michelle; Al-Riyami, Lamyaa; Rzepecka, Justyna; Harnett, William; Harnett, Margaret .

In: Journal of Parasitology Research, Vol. 2013, 961268, 2013.

Research output: Contribution to journalArticle

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