Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts

S J Cook, S Palmer, R Plevin, M J Wakelam

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.

Original languageEnglish
Pages (from-to)617-620
Number of pages4
JournalBiochemical journal
Volume265
Issue number2
Publication statusPublished - 15 Jan 1990

Keywords

  • adenosine triphosphate
  • adrenal cortex
  • animals
  • bombesin
  • calcium channels
  • cattle
  • cell membrane
  • cells, cultured
  • diacylglycerol kinase
  • diglycerides
  • fibroblasts
  • glycerides
  • inositol 1,4,5-trisphosphate
  • inositol 1,4,5-trisphosphate receptors
  • kinetics
  • mice
  • phosphorus radioisotopes
  • phosphotransferases
  • radioisotope dilution technique
  • receptors, cell surface
  • receptors, cytoplasmic and nuclear
  • tritium

Fingerprint Dive into the research topics of 'Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts'. Together they form a unique fingerprint.

  • Cite this