Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts

S J Cook, S Palmer, R Plevin, M J Wakelam

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.

Original languageEnglish
Pages (from-to)617-620
Number of pages4
JournalBiochemical journal
Volume265
Issue number2
Publication statusPublished - 15 Jan 1990

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Bombesin
Inositol 1,4,5-Trisphosphate
Fibroblasts
Swiss 3T3 Cells
Diglycerides
Assays
1,2-diacylglycerol

Keywords

  • adenosine triphosphate
  • adrenal cortex
  • animals
  • bombesin
  • calcium channels
  • cattle
  • cell membrane
  • cells, cultured
  • diacylglycerol kinase
  • diglycerides
  • fibroblasts
  • glycerides
  • inositol 1,4,5-trisphosphate
  • inositol 1,4,5-trisphosphate receptors
  • kinetics
  • mice
  • phosphorus radioisotopes
  • phosphotransferases
  • radioisotope dilution technique
  • receptors, cell surface
  • receptors, cytoplasmic and nuclear
  • tritium

Cite this

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title = "Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts",
abstract = "Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.",
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Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts. / Cook, S J; Palmer, S; Plevin, R; Wakelam, M J.

In: Biochemical journal, Vol. 265, No. 2, 15.01.1990, p. 617-620.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts

AU - Cook, S J

AU - Palmer, S

AU - Plevin, R

AU - Wakelam, M J

PY - 1990/1/15

Y1 - 1990/1/15

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AB - Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.

KW - adenosine triphosphate

KW - adrenal cortex

KW - animals

KW - bombesin

KW - calcium channels

KW - cattle

KW - cell membrane

KW - cells, cultured

KW - diacylglycerol kinase

KW - diglycerides

KW - fibroblasts

KW - glycerides

KW - inositol 1,4,5-trisphosphate

KW - inositol 1,4,5-trisphosphate receptors

KW - kinetics

KW - mice

KW - phosphorus radioisotopes

KW - phosphotransferases

KW - radioisotope dilution technique

KW - receptors, cell surface

KW - receptors, cytoplasmic and nuclear

KW - tritium

UR - http://www.biochemj.org/bj/265/bj2650617.htm

M3 - Article

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JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 2

ER -