Magnetic resonance imaging (MRI) of heavy-metal transport and fate in an artificial biofilm

V. R. Phoenix, W. M. Holmes, B. Ramanan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Unlike planktonic systems, reaction rates in biofilms are often limited by mass transport, which controls the rate of supply of contaminants into the biofilm matrix. To help understand this phenomenon, we investigated the potential of magnetic resonance imaging (MRI) to spatially quantify copper transport and fate in biofilms. For this initial study we utilized an artificial biofilm composed of a 50:50 mix of bacteria and agar. MRI successfully mapped Cu2+ uptake into the artificial biofilm by mapping T2 relaxation rates. A calibration protocol was used to convert T2 values into actual copper concentrations. Immobilization rates in the artificial biofilm were slow compared to the rapid equilibration of planktonic systems. Even after 36 h, the copper front had migrated only 3 mm into the artificial biofilm and at this distance from the copper source, concentrations were very low. This slow equilibration is a result of (1) the time it takes copper to diffuse over such distances and (2) the adsorption of copper onto cell surfaces, which further impedes copper diffusion. The success of this trial run indicates MRI could be used to quantitatively map heavy metal transport and immobilization in natural biofilms.

Original languageEnglish
Pages (from-to)483-486
Number of pages4
JournalMineralogical Magazine
Volume72
Issue number1
DOIs
Publication statusPublished - 29 Feb 2008
Externally publishedYes

Keywords

  • biofilm
  • magnetic resonance imaging
  • MRI
  • heavy metal transport

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