Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery

Supapitch Uten; Poramin Boonbanjong; Yosaphon Prueksathaporn; Kiatnida Treerattrakoon; Nuankanya Sathirapongsasuti; Narong Chanlek; Supree Pinitsoontorn; Patraporn Luksirikul; Deanpen Japrung

doi:10.1021/acsomega.3c05919

Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery

Supapitch Uten, Poramin Boonbanjong, Yosaphon Prueksathaporn, Kiatnida Treerattrakoon, Nuankanya Sathirapongsasuti, Narong Chanlek, Supree Pinitsoontorn, Patraporn Luksirikul^*, Deanpen Japrung^*

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

15 Downloads (Pure)

Abstract

In this study, we developed magnetic graphene oxide composites by chemically attaching Fe₃O₄ nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe₃O₄@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.

Original language	English
Pages (from-to)	2263-2271
Number of pages	9
Journal	ACS Omega
Volume	9
Issue number	2
Early online date	2 Jan 2024
DOIs	https://doi.org/10.1021/acsomega.3c05919
Publication status	Published - 16 Jan 2024

Funding

The authors gratefully acknowledge the financial support and the facilities provided by the National Nanotechnology Center (NANOTEC), the Research Network NANOTEC-KU (RNN), the Kasetsart University Research Development Institute (KURDI), and Synchrotron Light Research Institute for experimental instruments. P.B. thanks the Thailand Graduate Institute of Science and Technology (TGIST) and NSTDA for their support. K.T. thanks the National Science and Technology Development Agency and the University of Strathclyde for the award of a joint research degree.

Keywords

composites
nanoparticles
oxides
two dimensional materials
urea

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10.1021/acsomega.3c05919Licence: CC BY-NC-ND 4.0

Uten-etal-ACS-Omega-2024-Magnetic-graphene-oxide-nanocomposites-for-selective-miRNA-separationFinal published version, 4.63 MBLicence: CC BY-NC-ND 4.0

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title = "Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery",

abstract = "In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.",

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author = "Supapitch Uten and Poramin Boonbanjong and Yosaphon Prueksathaporn and Kiatnida Treerattrakoon and Nuankanya Sathirapongsasuti and Narong Chanlek and Supree Pinitsoontorn and Patraporn Luksirikul and Deanpen Japrung",

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AU - Uten, Supapitch

AU - Boonbanjong, Poramin

AU - Prueksathaporn, Yosaphon

AU - Treerattrakoon, Kiatnida

AU - Sathirapongsasuti, Nuankanya

AU - Chanlek, Narong

AU - Pinitsoontorn, Supree

AU - Luksirikul, Patraporn

AU - Japrung, Deanpen

PY - 2024/1/16

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N2 - In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.

AB - In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.

KW - composites

KW - nanoparticles

KW - oxides

KW - two dimensional materials

KW - urea

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JO - ACS Omega

JF - ACS Omega

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Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery

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