Magnetic graphene oxide nanocomposites for selective miRNA separation and recovery

Supapitch Uten, Poramin Boonbanjong, Yosaphon Prueksathaporn, Kiatnida Treerattrakoon, Nuankanya Sathirapongsasuti, Narong Chanlek, Supree Pinitsoontorn, Patraporn Luksirikul*, Deanpen Japrung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
2 Downloads (Pure)


In this study, we developed magnetic graphene oxide composites by chemically attaching Fe3O4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe3O4@GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.

Original languageEnglish
Pages (from-to)2263-2271
Number of pages9
JournalACS Omega
Issue number2
Early online date2 Jan 2024
Publication statusPublished - 16 Jan 2024


  • composites
  • nanoparticles
  • oxides
  • two dimensional materials
  • urea


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