Projects per year
Abstract
Phase microscopy allows stain-free imaging of transparent biological samples. One technique, using the transport of intensity equation (TIE), can be performed without dedicated hardware by simply processing pairs of images taken at known spacings within the sample. The resulting TIE images are quantitative phase maps of unstained biological samples. Therefore, spatially resolved optical path length (OPL) information can also be determined. Using low-cost, opensource hardware, we applied the TIE to living algal cells to measure their effect on OPL. We obtained OPL values that were repeatable within species and differed by distinct amounts depending on the species being measured. We suggest TIE imaging as a method of discrimination between different algal species and, potentially, non-biological materials, based on refractive index/OPL. Potential applications in biogeochemical modelling and climate sciences are suggested.
Original language | English |
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Article number | 191921 |
Number of pages | 12 |
Journal | Royal Society Open Science |
Volume | 7 |
Issue number | 1 |
DOIs | |
Publication status | Published - 29 Jan 2020 |
Keywords
- algeu
- phase-microscopy
- three-dimensional printing
- 3D printing
- open access
- ecosystems
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Dive into the research topics of 'Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation'. Together they form a unique fingerprint.Projects
- 1 Finished
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Low-cost, open-access imaging for identifying and quantifying water quality (GCRF)
Patton, B. (Principal Investigator)
4/12/17 → 30/04/19
Project: Research
Datasets
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Data for: "Low-cost, open-access quantitative phase imaging of algal cells using the transport of intensity equation"
Grant, S. (Creator), Patton, B. (Creator), McKee, D. (Creator) & Burdett, H. (Creator), University of Strathclyde, 21 Jan 2020
DOI: 10.15129/69626e13-2fb1-4ed2-9b1b-70bfb938fe91
Dataset