Loss of Gli3 enhances the viability of embryonic telencephalic cells in vitro

Paulett A Zaki, Ben Martynoga, J T Delafield-Butt, V Fotaki, T Yu, D J Price

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The transcription factor Gli3 is important for brain and limb development. Mice homozygous for a mutation in Gli3 (Gli3(Xt/Xt)) have severe abnormalities of telencephalic development and previous studies have suggested that aberrant cell death may contribute to the Gli3(Xt/Xt) phenotype. We demonstrate that telencephalic cells from embryonic Gli3(Xt/Xt) embryos survive better and are more resistant to death induced by cytosine arabinoside, a nucleoside analogue that induces death in neuronal progenitors and neurons, than are control counterparts in vitro. Culture medium conditioned by Gli3(Xt/Xt) cells is more effective at enhancing the viability of control telencephalic cells than medium conditioned by control cells, indicating that Gli3(Xt/Xt) cells release a factor or factors which enhance telencephalic cell viability. Gli3(Xt/Xt) cells are also more sensitive to released factors present in conditioned media. These data suggest that Gli3 plays both cell-autonomous and cell-nonautonomous roles in mediating telencephalic cell viability.

Original languageEnglish
Pages (from-to)1547-1551
Number of pages5
JournalEuropean Journal of Neuroscience
Volume22
Issue number6
DOIs
Publication statusPublished - Sep 2005

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Keywords

  • cell death
  • mouse
  • primary culture

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