Long-range surface plasmon resonance imaging for bioaffinity sensing

A.W. Wark, H.J. Lee, R.M. Corn

Research output: Contribution to journalArticle

206 Citations (Scopus)


A novel bioaffinity sensor based on surface plasmon resonance (SPR) imaging measurements of a multiple-layered structure that supports the generation of long-range surface plasmons (LRSPs) at the water−metal interface is reported. LRSPs possess longer surface propagation lengths, higher electric field strengths, and sharper angular resonance curves than conventional surface plasmons. LRSPR imaging is a version of SPR imaging that requires a symmetric dielectric arrangement around the gold thin film. This arrangement is created using an SF10 prism/Cytop/gold/water multilayer film structure where Cytop is an amorphous fluoropolymer with a refractive index very close to that of water. LRSPR imaging experiments are performed at a fixed incident angle and lead to an enhanced response for the detection of surface binding interactions. As an example, the hybridization adsorption of a 16-mer single-stranded DNA (ssDNA) onto a two-component ssDNA array was monitored with LRSPR imaging. The ssDNA array was created using a new fabrication technology appropriate for the LRSPR multilayers.
Original languageEnglish
Pages (from-to)3904-3907
Number of pages4
JournalAnalytical Chemistry
Issue number13
Early online date7 May 2005
Publication statusPublished - 2005


  • surface plasmon resonance
  • long-range surface plasmons
  • LRSPs
  • imaging
  • bioaffinity sensors

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