Local detection of enzymatic ion generation with polycrystalline silicon interdigitated electrodes and its application to biosensing

Roberto De La Rica, Antonio Baldi, Cesar Fernandez-Sanchez

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Abstract

Chips containing polycrystalline silicon interdigitated electrodes are modified with the enzyme urease. The sensors are able to detect changes in the resitivity of the solution near their surface, where the enzymatic reaction generates charged species. The electrodes are also grafted with an antigen and queried with different amounts of urease labeled antibody. The response of the modified electrodes is proportional to the amount of enzyme attached to the surface by the biorecognition event, thus validating the assay for biosensing applications.
Original languageEnglish
Article number074102
Number of pages4
JournalApplied Physics Letters
Volume90
DOIs
Publication statusPublished - 2007

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Keywords

  • local detection
  • enzymatic ion generation
  • polycrystalline silicon
  • biosensing
  • interdigitated electrodes

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