Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids

Ruth Welti, Ernie Mui, Alexis Sparks, Sarah Wernimont, Giorgis Isaac, Michael Kirisits, Mary Roth, Craic W. Roberts, Cyrille Botte, Eric Marechal, Rima McLeod

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Analysis of the polar lipids of Toxoplasma gondii by electrospray ionization tandem mass spectrometry provides a detailed picture of the lipid molecular species of this parasitic protozoan. Most notably, T gondii contains a relatively high level, estimated to about 2% of the total polar lipid, of ceramide phosphoethanolamine. The ceramide phosphoethanolamine has a fatty amide profile with only 16- and 18-carbon species. Compared with the host fibroblasts in which it was grown, T gondii also has higher levels of phosphatidylcholine but lower levels of sphingomyelin and phosphatidylserine. Analysis at the molecular species level indicated that T gondii has greater amounts of shorter-chain fatty acid in its polar lipid molecular species than the host fibroblasts. Shorter-chain fatty acids with a combined total of 30 or fewer acyl carbons make up 21% of Toxoplasma's, but only 3% of the host's, diacyl phosphatidylcholine. Furthermore, diacyl phosphatidylcholine with two saturated acyl chains with 12, 14, or 16 carbons make up over 11% of parasite phosphatidylcholine but less than 3% of the host phosphatidylcholine molecular species. The distinctive T. gondii tachyzoite lipid profile may be particularly suited to the function of parasitic membranes and the interaction of the parasite with the host cell and the host's immune system. Combined with T gondii genomic data, these lipidomic data will assist in elucidation of metabolic pathways for lipid biosynthesis in this important human pathogen.

LanguageEnglish
Pages13882-13890
Number of pages9
JournalBMC Biochemistry
Volume46
Issue number48
DOIs
Publication statusPublished - 4 Dec 2007

Fingerprint

Toxoplasma
Phosphatidylcholines
Lipids
Carbon
Volatile Fatty Acids
Fibroblasts
Host-Parasite Interactions
Electrospray ionization
Sphingomyelins
Electrospray Ionization Mass Spectrometry
Immune system
Phosphatidylserines
Biosynthesis
Pathogens
Tandem Mass Spectrometry
Metabolic Networks and Pathways
Amides
Mass spectrometry
Immune System
Parasites

Keywords

  • tandem mass-spectrometry
  • fatty-acid synthesis
  • ceramide-phosphorylethanolamine
  • plasmodium-falciparum
  • electrospray-ionization
  • apicomplexan parasites
  • membrane-lipids
  • host
  • metabolism
  • apicoplast

Cite this

Welti, R., Mui, E., Sparks, A., Wernimont, S., Isaac, G., Kirisits, M., ... McLeod, R. (2007). Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids. BMC Biochemistry, 46(48), 13882-13890. https://doi.org/10.1021/bi7011993
Welti, Ruth ; Mui, Ernie ; Sparks, Alexis ; Wernimont, Sarah ; Isaac, Giorgis ; Kirisits, Michael ; Roth, Mary ; Roberts, Craic W. ; Botte, Cyrille ; Marechal, Eric ; McLeod, Rima. / Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids. In: BMC Biochemistry. 2007 ; Vol. 46, No. 48. pp. 13882-13890.
@article{3463cb964f9e49859d160c43dbaaea20,
title = "Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids",
abstract = "Analysis of the polar lipids of Toxoplasma gondii by electrospray ionization tandem mass spectrometry provides a detailed picture of the lipid molecular species of this parasitic protozoan. Most notably, T gondii contains a relatively high level, estimated to about 2{\%} of the total polar lipid, of ceramide phosphoethanolamine. The ceramide phosphoethanolamine has a fatty amide profile with only 16- and 18-carbon species. Compared with the host fibroblasts in which it was grown, T gondii also has higher levels of phosphatidylcholine but lower levels of sphingomyelin and phosphatidylserine. Analysis at the molecular species level indicated that T gondii has greater amounts of shorter-chain fatty acid in its polar lipid molecular species than the host fibroblasts. Shorter-chain fatty acids with a combined total of 30 or fewer acyl carbons make up 21{\%} of Toxoplasma's, but only 3{\%} of the host's, diacyl phosphatidylcholine. Furthermore, diacyl phosphatidylcholine with two saturated acyl chains with 12, 14, or 16 carbons make up over 11{\%} of parasite phosphatidylcholine but less than 3{\%} of the host phosphatidylcholine molecular species. The distinctive T. gondii tachyzoite lipid profile may be particularly suited to the function of parasitic membranes and the interaction of the parasite with the host cell and the host's immune system. Combined with T gondii genomic data, these lipidomic data will assist in elucidation of metabolic pathways for lipid biosynthesis in this important human pathogen.",
keywords = "tandem mass-spectrometry, fatty-acid synthesis, ceramide-phosphorylethanolamine, plasmodium-falciparum, electrospray-ionization, apicomplexan parasites, membrane-lipids, host, metabolism, apicoplast",
author = "Ruth Welti and Ernie Mui and Alexis Sparks and Sarah Wernimont and Giorgis Isaac and Michael Kirisits and Mary Roth and Roberts, {Craic W.} and Cyrille Botte and Eric Marechal and Rima McLeod",
year = "2007",
month = "12",
day = "4",
doi = "10.1021/bi7011993",
language = "English",
volume = "46",
pages = "13882--13890",
journal = "BMC Biochemistry",
issn = "1471-2091",
number = "48",

}

Welti, R, Mui, E, Sparks, A, Wernimont, S, Isaac, G, Kirisits, M, Roth, M, Roberts, CW, Botte, C, Marechal, E & McLeod, R 2007, 'Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids' BMC Biochemistry, vol. 46, no. 48, pp. 13882-13890. https://doi.org/10.1021/bi7011993

Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids. / Welti, Ruth; Mui, Ernie; Sparks, Alexis; Wernimont, Sarah; Isaac, Giorgis; Kirisits, Michael; Roth, Mary; Roberts, Craic W.; Botte, Cyrille; Marechal, Eric; McLeod, Rima.

In: BMC Biochemistry, Vol. 46, No. 48, 04.12.2007, p. 13882-13890.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids

AU - Welti, Ruth

AU - Mui, Ernie

AU - Sparks, Alexis

AU - Wernimont, Sarah

AU - Isaac, Giorgis

AU - Kirisits, Michael

AU - Roth, Mary

AU - Roberts, Craic W.

AU - Botte, Cyrille

AU - Marechal, Eric

AU - McLeod, Rima

PY - 2007/12/4

Y1 - 2007/12/4

N2 - Analysis of the polar lipids of Toxoplasma gondii by electrospray ionization tandem mass spectrometry provides a detailed picture of the lipid molecular species of this parasitic protozoan. Most notably, T gondii contains a relatively high level, estimated to about 2% of the total polar lipid, of ceramide phosphoethanolamine. The ceramide phosphoethanolamine has a fatty amide profile with only 16- and 18-carbon species. Compared with the host fibroblasts in which it was grown, T gondii also has higher levels of phosphatidylcholine but lower levels of sphingomyelin and phosphatidylserine. Analysis at the molecular species level indicated that T gondii has greater amounts of shorter-chain fatty acid in its polar lipid molecular species than the host fibroblasts. Shorter-chain fatty acids with a combined total of 30 or fewer acyl carbons make up 21% of Toxoplasma's, but only 3% of the host's, diacyl phosphatidylcholine. Furthermore, diacyl phosphatidylcholine with two saturated acyl chains with 12, 14, or 16 carbons make up over 11% of parasite phosphatidylcholine but less than 3% of the host phosphatidylcholine molecular species. The distinctive T. gondii tachyzoite lipid profile may be particularly suited to the function of parasitic membranes and the interaction of the parasite with the host cell and the host's immune system. Combined with T gondii genomic data, these lipidomic data will assist in elucidation of metabolic pathways for lipid biosynthesis in this important human pathogen.

AB - Analysis of the polar lipids of Toxoplasma gondii by electrospray ionization tandem mass spectrometry provides a detailed picture of the lipid molecular species of this parasitic protozoan. Most notably, T gondii contains a relatively high level, estimated to about 2% of the total polar lipid, of ceramide phosphoethanolamine. The ceramide phosphoethanolamine has a fatty amide profile with only 16- and 18-carbon species. Compared with the host fibroblasts in which it was grown, T gondii also has higher levels of phosphatidylcholine but lower levels of sphingomyelin and phosphatidylserine. Analysis at the molecular species level indicated that T gondii has greater amounts of shorter-chain fatty acid in its polar lipid molecular species than the host fibroblasts. Shorter-chain fatty acids with a combined total of 30 or fewer acyl carbons make up 21% of Toxoplasma's, but only 3% of the host's, diacyl phosphatidylcholine. Furthermore, diacyl phosphatidylcholine with two saturated acyl chains with 12, 14, or 16 carbons make up over 11% of parasite phosphatidylcholine but less than 3% of the host phosphatidylcholine molecular species. The distinctive T. gondii tachyzoite lipid profile may be particularly suited to the function of parasitic membranes and the interaction of the parasite with the host cell and the host's immune system. Combined with T gondii genomic data, these lipidomic data will assist in elucidation of metabolic pathways for lipid biosynthesis in this important human pathogen.

KW - tandem mass-spectrometry

KW - fatty-acid synthesis

KW - ceramide-phosphorylethanolamine

KW - plasmodium-falciparum

KW - electrospray-ionization

KW - apicomplexan parasites

KW - membrane-lipids

KW - host

KW - metabolism

KW - apicoplast

U2 - 10.1021/bi7011993

DO - 10.1021/bi7011993

M3 - Article

VL - 46

SP - 13882

EP - 13890

JO - BMC Biochemistry

T2 - BMC Biochemistry

JF - BMC Biochemistry

SN - 1471-2091

IS - 48

ER -

Welti R, Mui E, Sparks A, Wernimont S, Isaac G, Kirisits M et al. Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids. BMC Biochemistry. 2007 Dec 4;46(48):13882-13890. https://doi.org/10.1021/bi7011993