Linamarase activities in Bacillus spp. responsible for aerobic digestion

B. McNeil, L.M. Harvey, J.O. Ugwuanyi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Thermophilic Bacillus spp. isolated from thermophilic aerobic digestion (TAD) of model agricultural slurry were screened for ability to secret linamarase activity and degrade linamarin, a cyanogenic glycoside toxin abundant in cassava. Screening was performed by both linamarin - picrate assay and by p-nitrophenyl β-d-glucoside (PNPG) degradation, and results of both assays were related. Linamarase positive isolates were identified as Bacillus coagulans, Bacillus licheniformis and Bacillus stearothermophilus. Enzyme production was growth related and peak production was reached in 48 h in B. coagulans and 36 h in B. stearothermophilus. B. coagulans produced over 40 times greater activity than B. stearothermophilus. Enzyme productivity in shake flask was not strictly related to screening assay result. Crude enzyme of B. coagulans was optimally active at 75 °C while that of B. stearothermophilus was optimally active at 80 °C and both had optimum activity at pH 8.0. The thermophilic and neutrophilic- to marginally alkaline activity of the crude enzymes could be very useful in the detoxification and reprocessing of cyanogens containing cassava wastes by TAD for use in animal nutrition.
LanguageEnglish
Pages1501-1508
Number of pages7
JournalWaste Management
Volume27
Issue number11
DOIs
Publication statusPublished - 2007

Fingerprint

digestion
enzyme
cassava
assay
detoxification
toxin
slurry
productivity
degradation
screening

Keywords

  • reprocessing
  • detoxification
  • toxin
  • modeling
  • agricultural waste
  • aerobic digestion
  • thermophily
  • pharmacology

Cite this

McNeil, B. ; Harvey, L.M. ; Ugwuanyi, J.O. / Linamarase activities in Bacillus spp. responsible for aerobic digestion. In: Waste Management. 2007 ; Vol. 27, No. 11. pp. 1501-1508.
@article{442199504cd243dfb402135c8b75e259,
title = "Linamarase activities in Bacillus spp. responsible for aerobic digestion",
abstract = "Thermophilic Bacillus spp. isolated from thermophilic aerobic digestion (TAD) of model agricultural slurry were screened for ability to secret linamarase activity and degrade linamarin, a cyanogenic glycoside toxin abundant in cassava. Screening was performed by both linamarin - picrate assay and by p-nitrophenyl β-d-glucoside (PNPG) degradation, and results of both assays were related. Linamarase positive isolates were identified as Bacillus coagulans, Bacillus licheniformis and Bacillus stearothermophilus. Enzyme production was growth related and peak production was reached in 48 h in B. coagulans and 36 h in B. stearothermophilus. B. coagulans produced over 40 times greater activity than B. stearothermophilus. Enzyme productivity in shake flask was not strictly related to screening assay result. Crude enzyme of B. coagulans was optimally active at 75 °C while that of B. stearothermophilus was optimally active at 80 °C and both had optimum activity at pH 8.0. The thermophilic and neutrophilic- to marginally alkaline activity of the crude enzymes could be very useful in the detoxification and reprocessing of cyanogens containing cassava wastes by TAD for use in animal nutrition.",
keywords = "reprocessing, detoxification, toxin, modeling, agricultural waste, aerobic digestion, thermophily, pharmacology",
author = "B. McNeil and L.M. Harvey and J.O. Ugwuanyi",
year = "2007",
doi = "10.1016/j.wasman.2006.10.020",
language = "English",
volume = "27",
pages = "1501--1508",
journal = "Waste Management",
issn = "0956-053X",
number = "11",

}

Linamarase activities in Bacillus spp. responsible for aerobic digestion. / McNeil, B.; Harvey, L.M.; Ugwuanyi, J.O.

In: Waste Management, Vol. 27, No. 11, 2007, p. 1501-1508.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Linamarase activities in Bacillus spp. responsible for aerobic digestion

AU - McNeil, B.

AU - Harvey, L.M.

AU - Ugwuanyi, J.O.

PY - 2007

Y1 - 2007

N2 - Thermophilic Bacillus spp. isolated from thermophilic aerobic digestion (TAD) of model agricultural slurry were screened for ability to secret linamarase activity and degrade linamarin, a cyanogenic glycoside toxin abundant in cassava. Screening was performed by both linamarin - picrate assay and by p-nitrophenyl β-d-glucoside (PNPG) degradation, and results of both assays were related. Linamarase positive isolates were identified as Bacillus coagulans, Bacillus licheniformis and Bacillus stearothermophilus. Enzyme production was growth related and peak production was reached in 48 h in B. coagulans and 36 h in B. stearothermophilus. B. coagulans produced over 40 times greater activity than B. stearothermophilus. Enzyme productivity in shake flask was not strictly related to screening assay result. Crude enzyme of B. coagulans was optimally active at 75 °C while that of B. stearothermophilus was optimally active at 80 °C and both had optimum activity at pH 8.0. The thermophilic and neutrophilic- to marginally alkaline activity of the crude enzymes could be very useful in the detoxification and reprocessing of cyanogens containing cassava wastes by TAD for use in animal nutrition.

AB - Thermophilic Bacillus spp. isolated from thermophilic aerobic digestion (TAD) of model agricultural slurry were screened for ability to secret linamarase activity and degrade linamarin, a cyanogenic glycoside toxin abundant in cassava. Screening was performed by both linamarin - picrate assay and by p-nitrophenyl β-d-glucoside (PNPG) degradation, and results of both assays were related. Linamarase positive isolates were identified as Bacillus coagulans, Bacillus licheniformis and Bacillus stearothermophilus. Enzyme production was growth related and peak production was reached in 48 h in B. coagulans and 36 h in B. stearothermophilus. B. coagulans produced over 40 times greater activity than B. stearothermophilus. Enzyme productivity in shake flask was not strictly related to screening assay result. Crude enzyme of B. coagulans was optimally active at 75 °C while that of B. stearothermophilus was optimally active at 80 °C and both had optimum activity at pH 8.0. The thermophilic and neutrophilic- to marginally alkaline activity of the crude enzymes could be very useful in the detoxification and reprocessing of cyanogens containing cassava wastes by TAD for use in animal nutrition.

KW - reprocessing

KW - detoxification

KW - toxin

KW - modeling

KW - agricultural waste

KW - aerobic digestion

KW - thermophily

KW - pharmacology

UR - http://dx.doi.org/10.1016/j.wasman.2006.10.020

U2 - 10.1016/j.wasman.2006.10.020

DO - 10.1016/j.wasman.2006.10.020

M3 - Article

VL - 27

SP - 1501

EP - 1508

JO - Waste Management

T2 - Waste Management

JF - Waste Management

SN - 0956-053X

IS - 11

ER -