Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells

Jitkasem Meewan; Sukrut Somani; Partha Laskar; Craig Irving; Margaret Mullin; Stuart Woods; Craig W. Roberts; Abdullah R. Alzahrani; Valerie A. Ferro; Suzanne  McGill; Stefan  Weidt; Richard  Burchmore; Christine Dufès

doi:10.3390/pharmaceutics14020439

Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells

Jitkasem Meewan, Sukrut Somani, Partha Laskar, Craig Irving, Margaret Mullin, Stuart Woods, Craig W. Roberts, Abdullah R. Alzahrani, Valerie A. Ferro, Suzanne McGill, Stefan Weidt , Richard Burchmore , Christine Dufès

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Abstract

The formation of a protein layer "corona" on nanoparticle surface upon entry in a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrated that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy.

Original language	English
Article number	439
Number of pages	19
Journal	Pharmaceutics
Volume	14
Issue number	2
DOIs	https://doi.org/10.3390/pharmaceutics14020439
Publication status	Published - 18 Feb 2022

Funding

P.L. and S.S. are respectively funded by research grants from Worldwide Cancer Research [grant number 16?1303] and The Dunhill Medical Trust [grant number R463/0216]. A.R.A. is in receipt of a Ph.D. studentship from the Saudi Cultural Bureau and Umm Al-Qura University (Kingdom of Saudi Arabia). Funding: P.L. and S.S. are respectively funded by research grants from Worldwide Cancer Research [grant number 16–1303] and The Dunhill Medical Trust [grant number R463/0216]. A.R.A. is in receipt of a Ph.D. studentship from the Saudi Cultural Bureau and Umm Al-Qura University (Kingdom of Saudi Arabia).

Keywords

zein micelles
poly(ethylene glycol)
protein corona
cellular uptake
cancer cells
macrophages;
dendritic cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/pharmaceutics14020439Licence: CC BY 4.0

Meewan-etal-Pharmaceutics-2022-Limited-impact-of-the-protein-corona-on-the-cellular-uptake-of-PEGylated-zein-micellesFinal published version, 2.57 MBLicence: CC BY 4.0

Cite this

Meewan, J., Somani, S., Laskar, P., Irving, C., Mullin, M., Woods, S., Roberts, C. W., Alzahrani, A. R., Ferro, V. A., McGill, S., Weidt , S., Burchmore , R., & Dufès, C. (2022). Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells. Pharmaceutics, 14(2), Article 439. https://doi.org/10.3390/pharmaceutics14020439

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title = "Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells",

abstract = "The formation of a protein layer {"}corona{"} on nanoparticle surface upon entry in a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrated that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy.",

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author = "Jitkasem Meewan and Sukrut Somani and Partha Laskar and Craig Irving and Margaret Mullin and Stuart Woods and Roberts, \{Craig W.\} and Alzahrani, \{Abdullah R.\} and Ferro, \{Valerie A.\} and Suzanne McGill and Stefan Weidt and Richard Burchmore and Christine Duf{\`e}s",

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T1 - Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells

AU - Meewan, Jitkasem

AU - Somani, Sukrut

AU - Laskar, Partha

AU - Irving, Craig

AU - Mullin, Margaret

AU - Woods, Stuart

AU - Roberts, Craig W.

AU - Alzahrani, Abdullah R.

AU - Ferro, Valerie A.

AU - McGill, Suzanne

AU - Weidt , Stefan

AU - Burchmore , Richard

AU - Dufès, Christine

PY - 2022/2/18

Y1 - 2022/2/18

N2 - The formation of a protein layer "corona" on nanoparticle surface upon entry in a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrated that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy.

AB - The formation of a protein layer "corona" on nanoparticle surface upon entry in a biological environment was shown to strongly influence the interactions with cells, especially affecting the uptake of nanomedicines. In this work, we present the impact of the protein corona on the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. Zein was successfully conjugated with poly(ethylene glycol) (PEG) of varying chain lengths (5K and 10K) and assembled into micelles. Our results demonstrated that PEGylation conferred stealth effects to the zein micelles. The presence of human plasma did not impact the uptake levels of the micelles by melanoma cancer cells, regardless of the PEG chain length used. In contrast, it decreased the uptake by macrophages and dendritic cells. These results therefore make PEGylated zein micelles promising as potential drug delivery systems for cancer therapy.

KW - zein micelles

KW - poly(ethylene glycol)

KW - protein corona

KW - cellular uptake

KW - cancer cells

KW - macrophages;

KW - dendritic cells

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DO - 10.3390/pharmaceutics14020439

M3 - Article

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VL - 14

JO - Pharmaceutics

JF - Pharmaceutics

IS - 2

M1 - 439

ER -

Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells

Abstract

Funding

Keywords

UN SDGs

Access to Document

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Development of novel gene delivery systems for brain targeting by intravenous administration

Cite this

Limited impact of the protein corona on the cellular uptake of PEGylated zein micelles by melanoma cancer cells

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Development of novel gene delivery systems for brain targeting by intravenous administration

Cite this