Light-sheet microscopy beam path miniaturisation using optical MEMS

R. Bauer; S. Bakas; D. Uttamchandani

Abstract

Light-sheet microscopy (LSM) as 3D fluorescence imaging tool has shown impressive growth over the last decade, specifically with open-source and application tailored systems in mind. By structuring the fluorescence excitation light to only illuminate a thin sheet of the sample in the active focal plane of an orthogonal imaging path, low light doses and fast 3D imaging can be achieved. In this work, we present digital control of the light-sheet creation, positioning and imaging using Microelectromechanical Systems (MEMS) micromirrors and the use of their inherent miniaturisation potential.

Original language	English
Number of pages	1
Publication status	Published - 6 Sept 2018
Event	Photon 18 - Ashton University, Birmingham, United Kingdom Duration: 3 Sept 2018 → 6 Sept 2018 http://www.photon.org.uk/home

Conference

Conference	Photon 18
Country/Territory	United Kingdom
City	Birmingham
Period	3/09/18 → 6/09/18
Internet address	http://www.photon.org.uk/home

Keywords

MEMS
light-sheet microscopy
fluorescence

Access to Document

Bauer-etal-Photon2018-Light-sheet-microscopy-beam-path-miniaturisationAccepted author manuscript, 13.6 KB

Cite this

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title = "Light-sheet microscopy beam path miniaturisation using optical MEMS",

abstract = "Light-sheet microscopy (LSM) as 3D fluorescence imaging tool has shown impressive growth over the last decade, specifically with open-source and application tailored systems in mind. By structuring the fluorescence excitation light to only illuminate a thin sheet of the sample in the active focal plane of an orthogonal imaging path, low light doses and fast 3D imaging can be achieved. In this work, we present digital control of the light-sheet creation, positioning and imaging using Microelectromechanical Systems (MEMS) micromirrors and the use of their inherent miniaturisation potential.",

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author = "R. Bauer and S. Bakas and D. Uttamchandani",

year = "2018",

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T1 - Light-sheet microscopy beam path miniaturisation using optical MEMS

AU - Bauer, R.

AU - Bakas, S.

AU - Uttamchandani, D.

PY - 2018/9/6

Y1 - 2018/9/6

N2 - Light-sheet microscopy (LSM) as 3D fluorescence imaging tool has shown impressive growth over the last decade, specifically with open-source and application tailored systems in mind. By structuring the fluorescence excitation light to only illuminate a thin sheet of the sample in the active focal plane of an orthogonal imaging path, low light doses and fast 3D imaging can be achieved. In this work, we present digital control of the light-sheet creation, positioning and imaging using Microelectromechanical Systems (MEMS) micromirrors and the use of their inherent miniaturisation potential.

AB - Light-sheet microscopy (LSM) as 3D fluorescence imaging tool has shown impressive growth over the last decade, specifically with open-source and application tailored systems in mind. By structuring the fluorescence excitation light to only illuminate a thin sheet of the sample in the active focal plane of an orthogonal imaging path, low light doses and fast 3D imaging can be achieved. In this work, we present digital control of the light-sheet creation, positioning and imaging using Microelectromechanical Systems (MEMS) micromirrors and the use of their inherent miniaturisation potential.

KW - MEMS

KW - light-sheet microscopy

KW - fluorescence

UR - http://www.photon.org.uk/

M3 - Abstract

T2 - Photon 18

Y2 - 3 September 2018 through 6 September 2018

ER -

Light-sheet microscopy beam path miniaturisation using optical MEMS

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Miniaturised 3D-printed biomedical imaging system using optical MEMS - RAE Fellowships (GCRF)

MEMS micromirror based light sheet generator for biomedical imaging

Conference on Optical MEMS and Nanophotonics 2018

Cite this

Light-sheet microscopy beam path miniaturisation using optical MEMS

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Miniaturised 3D-printed biomedical imaging system using optical MEMS - RAE Fellowships (GCRF)

Research output

MEMS micromirror based light sheet generator for biomedical imaging

Activities

Conference on Optical MEMS and Nanophotonics 2018

Cite this