Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

Johanna Trägårdh; Gillian Robb; Kamal K.E. Gadalla; Stuart Cobb; Christopher Travis; Gian-Luca Oppo; Gail McConnell

doi:10.1364/OL.40.003484

Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

Johanna Trägårdh, Gillian Robb, Kamal K.E. Gadalla, Stuart Cobb, Christopher Travis, Gian-Luca Oppo, Gail McConnell

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Abstract

We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .

Original language	English
Journal	Optics Letters
DOIs	https://doi.org/10.1364/OL.40.003484
Publication status	Published - 21 Jul 2015

Keywords

label free imaging
optical parametric generator
wavelength
nonlinear microscopy
harmonic generation and mixing
parametric oscillators and amplifiers

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10.1364/OL.40.003484

Tragardh-etal-OL-2015-Label-free-imaging-of-thick-tissue-at-1550nm-using-a-femtosecondAccepted author manuscript, 463 KB

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title = "Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator",

abstract = "We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .",

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AU - Trägårdh, Johanna

AU - Robb, Gillian

AU - Gadalla, Kamal K.E.

AU - Cobb, Stuart

AU - Travis, Christopher

AU - Oppo, Gian-Luca

AU - McConnell, Gail

N1 - © 2015 Optical Society of America. One print or electroni c copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

PY - 2015/7/21

Y1 - 2015/7/21

N2 - We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .

AB - We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .

KW - label free imaging

KW - optical parametric generator

KW - wavelength

KW - nonlinear microscopy

KW - harmonic generation and mixing

KW - parametric oscillators and amplifiers

UR - https://www.osapublishing.org/ol/home.cfm

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DO - 10.1364/OL.40.003484

M3 - Article

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JF - Optics Letters

SN - 0146-9592

ER -

Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

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Keywords

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Gail McConnell

MESOLAB: A Centre For Optical Mesoscopy For Biomedical Research At The University Of Strathclyde

Super-resolution optical microscopy via nonlinear self-focusing