Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

Johanna Trägårdh, Gillian Robb, Kamal K.E. Gadalla, Stuart Cobb, Christopher Travis, Gian-Luca Oppo, Gail McConnell

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
LanguageEnglish
JournalOptics Letters
DOIs
Publication statusPublished - 21 Jul 2015

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generators
brain
blood vessels
lithium niobates
pulses
wavelengths
fiber lasers
vessels
harmonic generations
broadband
damage
microscopy
nuclei
decay
cells
crystals
energy

Keywords

  • label free imaging
  • optical parametric generator
  • wavelength
  • nonlinear microscopy
  • harmonic generation and mixing
  • parametric oscillators and amplifiers

Cite this

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title = "Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator",
abstract = "We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .",
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month = "7",
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Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator. / Trägårdh, Johanna; Robb, Gillian; Gadalla, Kamal K.E.; Cobb, Stuart; Travis, Christopher; Oppo, Gian-Luca; McConnell, Gail.

In: Optics Letters, 21.07.2015.

Research output: Contribution to journalArticle

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AU - Robb, Gillian

AU - Gadalla, Kamal K.E.

AU - Cobb, Stuart

AU - Travis, Christopher

AU - Oppo, Gian-Luca

AU - McConnell, Gail

N1 - © 2015 Optical Society of America. One print or electroni c copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

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