Projects per year
Abstract
We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
Original language | English |
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Journal | Optics Letters |
DOIs | |
Publication status | Published - 21 Jul 2015 |
Keywords
- label free imaging
- optical parametric generator
- wavelength
- nonlinear microscopy
- harmonic generation and mixing
- parametric oscillators and amplifiers
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Projects
- 2 Finished
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MESOLAB: A Centre For Optical Mesoscopy For Biomedical Research At The University Of Strathclyde
McConnell, G. & Dempster, J.
MRC (Medical Research Council)
13/02/13 → 12/02/20
Project: Research
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Super-resolution optical microscopy via nonlinear self-focusing
EPSRC (Engineering and Physical Sciences Research Council)
1/01/11 → 28/02/15
Project: Research
Datasets
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Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator
Trägårdh, J. (Creator), Robb, G. (Creator), Gadalla, K. (Creator), Cobb, S. (Creator), Travis, C. (Creator), Oppo, G. (Creator) & McConnell, G. (Creator), University of Strathclyde, 30 Jun 2015
DOI: 10.15129/4391ea7c-ad05-4e09-87ae-079009fd8cbc
Dataset