Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator

Johanna Trägårdh, Gillian Robb, Kamal K.E. Gadalla, Stuart Cobb, Christopher Travis, Gian-Luca Oppo, Gail McConnell

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We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
Original languageEnglish
JournalOptics Letters
Publication statusPublished - 21 Jul 2015


  • label free imaging
  • optical parametric generator
  • wavelength
  • nonlinear microscopy
  • harmonic generation and mixing
  • parametric oscillators and amplifiers

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