Abstract
| Language | English |
|---|---|
| Journal | Optics Letters |
| DOIs | |
| Publication status | Published - 21 Jul 2015 |
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Keywords
- label free imaging
- optical parametric generator
- wavelength
- nonlinear microscopy
- harmonic generation and mixing
- parametric oscillators and amplifiers
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Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator. / Trägårdh, Johanna; Robb, Gillian; Gadalla, Kamal K.E.; Cobb, Stuart; Travis, Christopher; Oppo, Gian-Luca; McConnell, Gail.
In: Optics Letters, 21.07.2015.Research output: Contribution to journal › Article
TY - JOUR
T1 - Label-free imaging of thick tissue at 1550nm using a femtosecond optical parametric generator
AU - Trägårdh, Johanna
AU - Robb, Gillian
AU - Gadalla, Kamal K.E.
AU - Cobb, Stuart
AU - Travis, Christopher
AU - Oppo, Gian-Luca
AU - McConnell, Gail
N1 - © 2015 Optical Society of America. One print or electroni c copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
PY - 2015/7/21
Y1 - 2015/7/21
N2 - We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
AB - We have developed a simple wavelength tunable optical parametric generator (OPG), emitting broad band ultrashort pulses with peak wavelengths at 1530-1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third harmonic generation, of nuclei of brain cells and blood vessels in a >150 µm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW .
KW - label free imaging
KW - optical parametric generator
KW - wavelength
KW - nonlinear microscopy
KW - harmonic generation and mixing
KW - parametric oscillators and amplifiers
UR - https://www.osapublishing.org/ol/home.cfm
U2 - 10.1364/OL.40.003484
DO - 10.1364/OL.40.003484
M3 - Article
JO - Optics Letters
T2 - Optics Letters
JF - Optics Letters
SN - 0146-9592
ER -