Label-free cancer cell detection with impedimetric transducers

Roberto De La Rica, Sebastian Thompson, Antoni Baldi, Cesar Fernandez-Sanchez, Charles Michael Drain, Hiroshi Matsui

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

While cancer is still an implacable disease, many cancers can be cured if they are diagnosed in an early stage. Recently, it was reported that the transformation from normal cells to cancer cells can change their mechano-elastic properties to become softer and more deformable. If some cancer cells are more deformable, then a progressive increase of the volume of softer cancer cells should be induced as an abrupt change in osmolarity is applied. On the basis of this hypothesis, we developed a sensor that can electronically monitor the volume increase of cancer cells under hyposmotic pressure. By this methodology, K:Molv NIH 3T3 cells, 786-O human kidney carcinoma cells, and MPSC-1 ovarian cancer cells were successfully detected within 30 min using on the order of 10 cells. These cancer cells could be detected with the same sensitivity even in the presence of a vast excess of the respective noncancerous cells [NIH 3T3 cells, human embryonic kidney (HEK) 293 cells, ovarian surface epithelial (OSE) cells]. Since the proposed impedimetric sensor could be useful for detecting cancer cells fast and reliably, it could be further implemented in the screening of large populations of tissue samples and the detection of circulating tumor cells for point-of-care applications.
LanguageEnglish
Pages10167–10171
Number of pages5
JournalAnalytical Chemistry
Volume81
Issue number24
DOIs
Publication statusPublished - 13 Nov 2009

Fingerprint

Labels
Transducers
transducers
cancer
Cells
cells
kidneys
Sensors
sensors
Tumors
Screening
tumors
screening
Tissue
elastic properties
methodology
sensitivity

Keywords

  • cancer cells
  • cancer detection
  • impedimetric transducers

Cite this

De La Rica, R., Thompson, S., Baldi, A., Fernandez-Sanchez, C., Drain, C. M., & Matsui, H. (2009). Label-free cancer cell detection with impedimetric transducers. Analytical Chemistry, 81(24), 10167–10171. https://doi.org/10.1021/ac9021049
De La Rica, Roberto ; Thompson, Sebastian ; Baldi, Antoni ; Fernandez-Sanchez, Cesar ; Drain, Charles Michael ; Matsui, Hiroshi. / Label-free cancer cell detection with impedimetric transducers. In: Analytical Chemistry. 2009 ; Vol. 81, No. 24. pp. 10167–10171.
@article{3d5964ede5a945abaf1b2c6a0b1bca7c,
title = "Label-free cancer cell detection with impedimetric transducers",
abstract = "While cancer is still an implacable disease, many cancers can be cured if they are diagnosed in an early stage. Recently, it was reported that the transformation from normal cells to cancer cells can change their mechano-elastic properties to become softer and more deformable. If some cancer cells are more deformable, then a progressive increase of the volume of softer cancer cells should be induced as an abrupt change in osmolarity is applied. On the basis of this hypothesis, we developed a sensor that can electronically monitor the volume increase of cancer cells under hyposmotic pressure. By this methodology, K:Molv NIH 3T3 cells, 786-O human kidney carcinoma cells, and MPSC-1 ovarian cancer cells were successfully detected within 30 min using on the order of 10 cells. These cancer cells could be detected with the same sensitivity even in the presence of a vast excess of the respective noncancerous cells [NIH 3T3 cells, human embryonic kidney (HEK) 293 cells, ovarian surface epithelial (OSE) cells]. Since the proposed impedimetric sensor could be useful for detecting cancer cells fast and reliably, it could be further implemented in the screening of large populations of tissue samples and the detection of circulating tumor cells for point-of-care applications.",
keywords = "cancer cells, cancer detection, impedimetric transducers",
author = "{De La Rica}, Roberto and Sebastian Thompson and Antoni Baldi and Cesar Fernandez-Sanchez and Drain, {Charles Michael} and Hiroshi Matsui",
year = "2009",
month = "11",
day = "13",
doi = "10.1021/ac9021049",
language = "English",
volume = "81",
pages = "10167–10171",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "24",

}

De La Rica, R, Thompson, S, Baldi, A, Fernandez-Sanchez, C, Drain, CM & Matsui, H 2009, 'Label-free cancer cell detection with impedimetric transducers' Analytical Chemistry, vol. 81, no. 24, pp. 10167–10171. https://doi.org/10.1021/ac9021049

Label-free cancer cell detection with impedimetric transducers. / De La Rica, Roberto; Thompson, Sebastian; Baldi, Antoni; Fernandez-Sanchez, Cesar; Drain, Charles Michael; Matsui, Hiroshi.

In: Analytical Chemistry, Vol. 81, No. 24, 13.11.2009, p. 10167–10171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Label-free cancer cell detection with impedimetric transducers

AU - De La Rica, Roberto

AU - Thompson, Sebastian

AU - Baldi, Antoni

AU - Fernandez-Sanchez, Cesar

AU - Drain, Charles Michael

AU - Matsui, Hiroshi

PY - 2009/11/13

Y1 - 2009/11/13

N2 - While cancer is still an implacable disease, many cancers can be cured if they are diagnosed in an early stage. Recently, it was reported that the transformation from normal cells to cancer cells can change their mechano-elastic properties to become softer and more deformable. If some cancer cells are more deformable, then a progressive increase of the volume of softer cancer cells should be induced as an abrupt change in osmolarity is applied. On the basis of this hypothesis, we developed a sensor that can electronically monitor the volume increase of cancer cells under hyposmotic pressure. By this methodology, K:Molv NIH 3T3 cells, 786-O human kidney carcinoma cells, and MPSC-1 ovarian cancer cells were successfully detected within 30 min using on the order of 10 cells. These cancer cells could be detected with the same sensitivity even in the presence of a vast excess of the respective noncancerous cells [NIH 3T3 cells, human embryonic kidney (HEK) 293 cells, ovarian surface epithelial (OSE) cells]. Since the proposed impedimetric sensor could be useful for detecting cancer cells fast and reliably, it could be further implemented in the screening of large populations of tissue samples and the detection of circulating tumor cells for point-of-care applications.

AB - While cancer is still an implacable disease, many cancers can be cured if they are diagnosed in an early stage. Recently, it was reported that the transformation from normal cells to cancer cells can change their mechano-elastic properties to become softer and more deformable. If some cancer cells are more deformable, then a progressive increase of the volume of softer cancer cells should be induced as an abrupt change in osmolarity is applied. On the basis of this hypothesis, we developed a sensor that can electronically monitor the volume increase of cancer cells under hyposmotic pressure. By this methodology, K:Molv NIH 3T3 cells, 786-O human kidney carcinoma cells, and MPSC-1 ovarian cancer cells were successfully detected within 30 min using on the order of 10 cells. These cancer cells could be detected with the same sensitivity even in the presence of a vast excess of the respective noncancerous cells [NIH 3T3 cells, human embryonic kidney (HEK) 293 cells, ovarian surface epithelial (OSE) cells]. Since the proposed impedimetric sensor could be useful for detecting cancer cells fast and reliably, it could be further implemented in the screening of large populations of tissue samples and the detection of circulating tumor cells for point-of-care applications.

KW - cancer cells

KW - cancer detection

KW - impedimetric transducers

U2 - 10.1021/ac9021049

DO - 10.1021/ac9021049

M3 - Article

VL - 81

SP - 10167

EP - 10171

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 24

ER -

De La Rica R, Thompson S, Baldi A, Fernandez-Sanchez C, Drain CM, Matsui H. Label-free cancer cell detection with impedimetric transducers. Analytical Chemistry. 2009 Nov 13;81(24):10167–10171. https://doi.org/10.1021/ac9021049