Kinase-inactivated ULK proteins inhibit autophagy via their conserved c-terminal domains using an Atg13-independent mechanism

Edmond Y. Chan, Andrea Longatti, Nicole C. McKnight, Sharon A. Tooze

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

The yeast Atg1 serine/threonine protein kinase and its mammalian homologs ULK1 and ULK2 play critical roles during the activation of autophagy. Previous studies have demonstrated that the conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Here, we explored the role of kinase activity and intramolecular interactions to further understand ULK function. We demonstrate that the dominant-negative activity of kinase-dead mutants requires a 7-residue motif within the CTD. Our data lead to a model in which the functions of ULK1 and ULK2 are controlled by autophosphorylation and conformational changes involving exposure of the CTD. Additional mapping indicates that the CTD contains other distinct regions that direct membrane association and interaction with the putative human homologue of Atg13, which we have here characterized. Atg13 is required for autophagy and Atg9 trafficking during autophagy. However, Atg13 does not bind the 7-residue dominant-negative motif in the CTD of ULK proteins nor is the inhibitory activity of the CTDs rescued by Atg13 ectopic expression, suggesting that in mammalian cells, the CTD may interact with additional autophagy proteins.
Original languageEnglish
Pages (from-to)157-171
Number of pages15
JournalMolecular and Cellular Biology
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2009

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Autophagy
Phosphotransferases
Proteins
Protein-Serine-Threonine Kinases
Yeasts
Membranes

Keywords

  • kinase-inactivated ULK proteins
  • autophagy
  • C-terminal domains
  • pharmacology
  • biomedical sciences

Cite this

Chan, Edmond Y. ; Longatti, Andrea ; McKnight, Nicole C. ; Tooze, Sharon A. / Kinase-inactivated ULK proteins inhibit autophagy via their conserved c-terminal domains using an Atg13-independent mechanism. In: Molecular and Cellular Biology. 2009 ; Vol. 29, No. 1. pp. 157-171.
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Kinase-inactivated ULK proteins inhibit autophagy via their conserved c-terminal domains using an Atg13-independent mechanism. / Chan, Edmond Y.; Longatti, Andrea; McKnight, Nicole C.; Tooze, Sharon A.

In: Molecular and Cellular Biology, Vol. 29, No. 1, 01.2009, p. 157-171.

Research output: Contribution to journalArticle

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