Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

Rhona Muir; Shareen Forbes; David J S Birch; Vladislav Vyshemirsky; Olaf J Rolinski

doi:10.1088/2050-6120/aca507

Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

Rhona Muir, Shareen Forbes, David J S Birch, Vladislav Vyshemirsky, Olaf J Rolinski

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Abstract

We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.

Original language	English
Article number	015003
Number of pages	22
Journal	Methods and Applications in Fluorescence
Volume	11
Issue number	1
Early online date	22 Nov 2022
DOIs	https://doi.org/10.1088/2050-6120/aca507
Publication status	Published - 1 Jan 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

keratin
glucose
protein glycation
intrinsic fluorescence
time-resolved fluorescence spectroscopy

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10.1088/2050-6120/aca507Licence: CC BY 4.0

Muir-etal-MAF-2022-Keratin-intrinsic-fluorescence-as-a-mechanism-for-non-invasive-monitoring-of-its-glycationFinal published version, 1.57 MBLicence: CC BY 4.0

Cite this

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title = "Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation",

abstract = "We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.",

keywords = "keratin, glucose, protein glycation, intrinsic fluorescence, time-resolved fluorescence spectroscopy",

author = "Rhona Muir and Shareen Forbes and Birch, \{David J S\} and Vladislav Vyshemirsky and Rolinski, \{Olaf J\}",

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T1 - Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

AU - Muir, Rhona

AU - Forbes, Shareen

AU - Birch, David J S

AU - Vyshemirsky, Vladislav

AU - Rolinski, Olaf J

PY - 2023/1/1

Y1 - 2023/1/1

N2 - We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.

AB - We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.

KW - keratin

KW - glucose

KW - protein glycation

KW - intrinsic fluorescence

KW - time-resolved fluorescence spectroscopy

U2 - 10.1088/2050-6120/aca507

DO - 10.1088/2050-6120/aca507

M3 - Article

SN - 2050-6120

VL - 11

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 1

M1 - 015003

ER -

Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

Abstract

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Keywords

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