Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation

Rhona Muir, Shareen Forbes, David J S Birch, Vladislav Vyshemirsky, Olaf J Rolinski

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Abstract

We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steadystate and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro. The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.
Original languageEnglish
Article number015003
Number of pages22
JournalMethods and Applications in Fluorescence
Volume11
Issue number1
Early online date22 Nov 2022
DOIs
Publication statusPublished - 1 Dec 2022

Keywords

  • keratin
  • glucose
  • protein glycation
  • intrinsic fluorescence
  • time-resolved fluorescence spectroscopy

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