Abstract
Isothermal amplification (IA) is a nucleic acid amplification technology (NAAT) that has contributed significantly to the healthcare system. The combination of NAAT with a suitable detection platform resulted in higher sensitivity, specificity, and rapid disease diagnosis. Traditional NAAT, such as polymerase chain reaction (PCR), is widely applied in the general healthcare system but is rarely accessed in resource-limited hospitals. Some IA methods provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been regularly used in clinical applications because different biomarkers and sample types affect either the enzyme in the IA system or sample preparation. This review focuses on the application of some IA techniques that have been applied in the medical field and have the potential for use at points of care.
| Original language | English |
|---|---|
| Article number | 677 |
| Journal | Biosensors |
| Volume | 12 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 24 Aug 2022 |
Funding
This review paper was funded by National Nanotechnology Center, National Science and Technology Development Agency (Grant no. P2150900 and P2250597). P.B. thanks the Thailand Graduate Institute of Science and Technology, K.T. thanks the National Science and Technology Development Agency, and the University of Strathclyde for the award of a joint research degree. P.P. acknowledges the support of the Royal Thai Government Scholarship Program by OCSC for Thai students to enroll in postgraduate programs in science and technology at the University of Chinese Academy of Science. The authors would like to thank Editage (accessed on 17 January 2022) for English language editing.
Keywords
- isothermal amplification
- nucleic acid amplification technology
- loop-mediated isothermal amplification
- rolling circle amplification
- CRISPR