Isothermal amplification technology for disease diagnosis

Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)
4 Downloads (Pure)

Abstract

Isothermal amplification (IA) is a nucleic acid amplification technology (NAAT) that has contributed significantly to the healthcare system. The combination of NAAT with a suitable detection platform resulted in higher sensitivity, specificity, and rapid disease diagnosis. Traditional NAAT, such as polymerase chain reaction (PCR), is widely applied in the general healthcare system but is rarely accessed in resource-limited hospitals. Some IA methods provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been regularly used in clinical applications because different biomarkers and sample types affect either the enzyme in the IA system or sample preparation. This review focuses on the application of some IA techniques that have been applied in the medical field and have the potential for use at points of care.
Original languageEnglish
Article number677
JournalBiosensors
Volume12
Issue number9
DOIs
Publication statusPublished - 24 Aug 2022

Keywords

  • isothermal amplification
  • nucleic acid amplification technology
  • loop-mediated isothermal amplification
  • rolling circle amplification
  • CRISPR

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