Isostructural replacement of zinc by cadmium in bacterial metallothionein

Claudia Blindauer, Mark Harrison, John Parkinson, Nigel Robinson, Peter Sadler

Research output: Contribution to journalArticle

Abstract

Abstract: The class II metallothionein SmtA from the cyanobacterium Synechococcus PCC 7942 plays a major role in providing tolerance to high levels of zinc as well as a lesser role in cadmium detoxification. We previously determined the NMR structure of Zn(4)SmtA [Blindauer et al., PNAS 2001, 98, 9593-9598] and assessed its zinc-binding dynamics. In the present report, we discuss the solution structure of cadmium-loaded SmtA and provide insight into the way in which cyanobacteria manage chemically similar toxic and essential metal ions. Comparisons of 3D structures and NMR data indicate that SmtA is able to accommodate either zinc or cadmium equally well. This work is not only relevant to understanding the biological role of bacterial metallothioneins, but also has implications for metalloprotein promiscuity and specificity.

Fingerprint

Metallothionein
Cadmium
Zinc
Nuclear magnetic resonance
Metalloproteins
Detoxification
Poisons
Metal ions
Cyanobacteria

Keywords

  • zinc
  • cadmium
  • detoxification
  • metal-ions
  • cyanobacteria
  • binding
  • locus

Cite this

Blindauer, Claudia ; Harrison, Mark ; Parkinson, John ; Robinson, Nigel ; Sadler, Peter. / Isostructural replacement of zinc by cadmium in bacterial metallothionein. In: Metal Ions in Biology and Medicine. 2008 ; Vol. 10. pp. 167-173.
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Blindauer, C, Harrison, M, Parkinson, J, Robinson, N & Sadler, P 2008, 'Isostructural replacement of zinc by cadmium in bacterial metallothionein' Metal Ions in Biology and Medicine, vol. 10, pp. 167-173.

Isostructural replacement of zinc by cadmium in bacterial metallothionein. / Blindauer, Claudia; Harrison, Mark; Parkinson, John; Robinson, Nigel; Sadler, Peter.

In: Metal Ions in Biology and Medicine, Vol. 10, 2008, p. 167-173.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isostructural replacement of zinc by cadmium in bacterial metallothionein

AU - Blindauer, Claudia

AU - Harrison, Mark

AU - Parkinson, John

AU - Robinson, Nigel

AU - Sadler, Peter

PY - 2008

Y1 - 2008

N2 - Abstract: The class II metallothionein SmtA from the cyanobacterium Synechococcus PCC 7942 plays a major role in providing tolerance to high levels of zinc as well as a lesser role in cadmium detoxification. We previously determined the NMR structure of Zn(4)SmtA [Blindauer et al., PNAS 2001, 98, 9593-9598] and assessed its zinc-binding dynamics. In the present report, we discuss the solution structure of cadmium-loaded SmtA and provide insight into the way in which cyanobacteria manage chemically similar toxic and essential metal ions. Comparisons of 3D structures and NMR data indicate that SmtA is able to accommodate either zinc or cadmium equally well. This work is not only relevant to understanding the biological role of bacterial metallothioneins, but also has implications for metalloprotein promiscuity and specificity.

AB - Abstract: The class II metallothionein SmtA from the cyanobacterium Synechococcus PCC 7942 plays a major role in providing tolerance to high levels of zinc as well as a lesser role in cadmium detoxification. We previously determined the NMR structure of Zn(4)SmtA [Blindauer et al., PNAS 2001, 98, 9593-9598] and assessed its zinc-binding dynamics. In the present report, we discuss the solution structure of cadmium-loaded SmtA and provide insight into the way in which cyanobacteria manage chemically similar toxic and essential metal ions. Comparisons of 3D structures and NMR data indicate that SmtA is able to accommodate either zinc or cadmium equally well. This work is not only relevant to understanding the biological role of bacterial metallothioneins, but also has implications for metalloprotein promiscuity and specificity.

KW - zinc

KW - cadmium

KW - detoxification

KW - metal-ions

KW - cyanobacteria

KW - binding

KW - locus

M3 - Article

VL - 10

SP - 167

EP - 173

JO - Metal Ions in Biology and Medicine

T2 - Metal Ions in Biology and Medicine

JF - Metal Ions in Biology and Medicine

SN - 1257-2535

ER -