Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment

A. Sakultantimetha, H.E. Keenan, M. Dyer, T.K. Beattie, S. Bangkedphol, A. Songsasen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tributyltin compound (TBT) released into the aquatic environment is generally degraded by bacteria in water and sediment. The isolation of TBT-degrading bacteria from TBT polluted sediment leads to the indication of specific potential TBT degraders. Two new strains of bacteria designated as B2 and B3 were successfully isolated using glycerol medium containing tributyltin chloride (TBTC) at 130 μM from contaminated sediment collected from Bowling Basin in Glasgow. The observed degradation after 14 days of the microcosm from the sediment and the isolated bacteria were investigated at an initial concentration of 1 μM TBTC. It was found that TBT was degraded by the bacterial strains B2 and B3 at 8.3 and 16.9 %, respectively. The results indicate that B2 and B3 are effective as TBT degraders. EC50 of B2 and B3 in water were 88.73 and 112.53 μM TBTC, which were significantly higher than the concentration of TBT measured at the basin, suggesting a low effect of TBT on the growth and activity of bacteria. After identification using API 20E and 16S sequencing, the bacterial isolate strain B2 is Citrobacter braakii and B3 is Enterobacter cloacae. Therefore, this study has discovered two species of high resistance TBT degrader which have never been previously studied or isolated based upon TBT degradation ability.
LanguageEnglish
Pages1-6
Number of pages5
JournalJournal of ASTM International
Volume6
Issue number6
DOIs
Publication statusPublished - 29 May 2009

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Citrobacter
Enterobacter cloacae
Bacteria
Sediments
Degradation
Glycerol
Application programming interfaces (API)
Water
tributyltin

Keywords

  • tributyltin
  • isolation
  • degradation
  • citrobacter braakii
  • enterobacter cloacae

Cite this

Sakultantimetha, A. ; Keenan, H.E. ; Dyer, M. ; Beattie, T.K. ; Bangkedphol, S. ; Songsasen, A. / Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment. In: Journal of ASTM International. 2009 ; Vol. 6, No. 6. pp. 1-6.
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Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment. / Sakultantimetha, A.; Keenan, H.E.; Dyer, M.; Beattie, T.K.; Bangkedphol, S.; Songsasen, A.

In: Journal of ASTM International, Vol. 6, No. 6, 29.05.2009, p. 1-6.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation of tributyltin-degrading bacteria citrobacter braakii and enterobacter cloacae from butyltin-polluted sediment

AU - Sakultantimetha, A.

AU - Keenan, H.E.

AU - Dyer, M.

AU - Beattie, T.K.

AU - Bangkedphol, S.

AU - Songsasen, A.

PY - 2009/5/29

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N2 - Tributyltin compound (TBT) released into the aquatic environment is generally degraded by bacteria in water and sediment. The isolation of TBT-degrading bacteria from TBT polluted sediment leads to the indication of specific potential TBT degraders. Two new strains of bacteria designated as B2 and B3 were successfully isolated using glycerol medium containing tributyltin chloride (TBTC) at 130 μM from contaminated sediment collected from Bowling Basin in Glasgow. The observed degradation after 14 days of the microcosm from the sediment and the isolated bacteria were investigated at an initial concentration of 1 μM TBTC. It was found that TBT was degraded by the bacterial strains B2 and B3 at 8.3 and 16.9 %, respectively. The results indicate that B2 and B3 are effective as TBT degraders. EC50 of B2 and B3 in water were 88.73 and 112.53 μM TBTC, which were significantly higher than the concentration of TBT measured at the basin, suggesting a low effect of TBT on the growth and activity of bacteria. After identification using API 20E and 16S sequencing, the bacterial isolate strain B2 is Citrobacter braakii and B3 is Enterobacter cloacae. Therefore, this study has discovered two species of high resistance TBT degrader which have never been previously studied or isolated based upon TBT degradation ability.

AB - Tributyltin compound (TBT) released into the aquatic environment is generally degraded by bacteria in water and sediment. The isolation of TBT-degrading bacteria from TBT polluted sediment leads to the indication of specific potential TBT degraders. Two new strains of bacteria designated as B2 and B3 were successfully isolated using glycerol medium containing tributyltin chloride (TBTC) at 130 μM from contaminated sediment collected from Bowling Basin in Glasgow. The observed degradation after 14 days of the microcosm from the sediment and the isolated bacteria were investigated at an initial concentration of 1 μM TBTC. It was found that TBT was degraded by the bacterial strains B2 and B3 at 8.3 and 16.9 %, respectively. The results indicate that B2 and B3 are effective as TBT degraders. EC50 of B2 and B3 in water were 88.73 and 112.53 μM TBTC, which were significantly higher than the concentration of TBT measured at the basin, suggesting a low effect of TBT on the growth and activity of bacteria. After identification using API 20E and 16S sequencing, the bacterial isolate strain B2 is Citrobacter braakii and B3 is Enterobacter cloacae. Therefore, this study has discovered two species of high resistance TBT degrader which have never been previously studied or isolated based upon TBT degradation ability.

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