Isolation and characterization of pyropheophorbide-a from moringa oleifera lam

Ukachi E. Igbo, John O. Igoli, Samuel O. Onyiriuka, Cynthia E. Ogukwe, Atu A. Ayuk, Alexander I. Gray

Research output: Contribution to journalArticle

Abstract

Moringa oleifera is a plant rich in pharmacologically active compounds. This study investigated the phytochemical constituents of Moringa oleifera leaf extracts. Dried M. oleifera leaves were ground and extracted successively with hexane, ethyl acetate and methanol using a Soxhlet apparatus. Different chromatographic techniques were used to fractionate the ethyl acetate extract. Thin layer chromatography was used to pool similar fractions together. Fractions obtained were purified using sephadex glass column. The structures of isolated compounds were identified using nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and comparison with published data. Phytochemical screening revealed the presence of flavonoids, terpenoids, carbohydrates and phenols. Ethyl acetate extract which was subjected to column chromatography resulted to the isolation of two compounds: mono acetyl glycerol and pyropheophorbide-a, from the leaves of M. oleifera. This is the first report on the isolation of pyropheophorbide-a from the leaves of M. oleifera.

Original languageEnglish
Pages (from-to)314-318
Number of pages5
JournalTropical Journal of Natural Product Research
Volume3
Issue number10
DOIs
Publication statusPublished - 10 Dec 2019

Keywords

  • isolation
  • monoacetyl glycerol
  • moringa oleifera
  • pyropheophorbide-a

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    Igbo, U. E., Igoli, J. O., Onyiriuka, S. O., Ogukwe, C. E., Ayuk, A. A., & Gray, A. I. (2019). Isolation and characterization of pyropheophorbide-a from moringa oleifera lam. Tropical Journal of Natural Product Research, 3(10), 314-318. https://doi.org/10.26538/tjnpr/v3i10.3