Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata

P. R. Haddrill, M. E N Majerus, S. Mayes

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Contrary to theoretical predictions, female 2-spot ladybirds (Adalia bipunctata) mate many more times than necessary to maintain high fertilisation success and may gain through the acquisition of material or genetic benefits. In order to investigate this mating system in detail, microsatellite markers have been isolated using a modified enrichment technique. Thirty-nine loci were successfully amplified by polymerase chain reaction (PCR), of which only two were monomorphic. Detailed characterization of ten loci revealed very high levels of polymorphism. These markers are likely to be invaluable tools with which to study population genetics and patterns of paternity in this species.

LanguageEnglish
Pages316-319
Number of pages4
JournalMolecular Ecology Notes
Volume2
Issue number3
Early online date22 Aug 2002
DOIs
Publication statusPublished - 1 Sep 2002

Fingerprint

Polymerase chain reaction
Population Genetics
Polymorphism
Fertilization
Microsatellite Repeats
Polymerase Chain Reaction
paternity
reproductive strategy
polymerase chain reaction
population genetics
Genes
polymorphism
prediction
marker
material

Keywords

  • 2-spot ladybird
  • adalia bipunctata
  • isolation and characterisation
  • highly polymorphic microsatellite loci
  • mating systems
  • paternity analysis
  • microsatellites
  • coccinellidae

Cite this

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Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata. / Haddrill, P. R.; Majerus, M. E N; Mayes, S.

In: Molecular Ecology Notes, Vol. 2, No. 3, 01.09.2002, p. 316-319.

Research output: Contribution to journalArticle

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